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Titolo:
Mechanisms of reduced SR Ca2+ release induced by inorganic phosphate in rat skeletal muscle fibers
Autore:
Duke, AM; Steele, DS;
Indirizzi:
Univ Leeds, Sch Biol, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT S2 9JT, W Yorkshire, England
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 2, volume: 281, anno: 2001,
pagine: C418 - C429
SICI:
0363-6143(200108)281:2<C418:MORSCR>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SARCOPLASMIC-RETICULUM; RYANODINE RECEPTOR; SKINNED SKELETAL; CALCIUM-RELEASE; SINGLE FIBERS; FK506-BINDING PROTEIN; ADENINE-NUCLEOTIDES; CREATINE-PHOSPHATE; XENOPUS-LAEVIS; LOW ATP;
Keywords:
calcium; magnesium; sarcoplasmic reticulum; fatigue;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Steele, DS Univ Leeds, Sch Biomed Sci, Leeds LS2 9JT, W Yorkshire, EnglandUniv Leeds Leeds W Yorkshire England LS2 9JT orkshire, England
Citazione:
A.M. Duke e D.S. Steele, "Mechanisms of reduced SR Ca2+ release induced by inorganic phosphate in rat skeletal muscle fibers", AM J P-CELL, 281(2), 2001, pp. C418-C429

Abstract

The effects of inorganic phosphate (P-i) on Ca2+ release from the sarcoplasmic reticulum (SR) were studied in mechanically skinned rat skeletal muscle fibers. Application of caffeine or T-tubule depolarization was used to induce Ca2+ release from the SR, which was detected using fura 2 fluorescence. Addition of P-i (1-40 mM) caused a reversible and concentration-dependentreduction in the caffeine-induced Ca2+ transient. This effect was apparentat low P-i concentration (<5 mM), which did not result in detectable precipitation of calcium phosphate within the SR. The inhibitory effect of P-i exhibited a marked dependence on free Mg2+ concentration. At 0.5 mM free Mg2, 5 mM P-i reduced the caffeine-induced transient by 25.1 <plus/minus> 4.1% (n = 13). However, at 1.5 mM free Mg2+, 5 mM P-i reduced the amplitude ofcaffeine-induced Ca2+ transients by 68.9 +/- 3.1% (n = 10). Depolarization-induced SR Ca2+ release was similarly affected. These effects of P-i may be important in skeletal muscle fatigue, if an inhibitory action of P-i on SR Ca2+ release is augmented by the rise in cytosolic Mg2+ concentration, which accompanies ATP breakdown.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 19:26:54