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Titolo:
Functionally distinct pools of actin in secretory cells
Autore:
Ammar, DA; Nguyen, PNB; Forte, JG;
Indirizzi:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 ll Biol, Berkeley, CA 94720 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 2, volume: 281, anno: 2001,
pagine: C407 - C417
SICI:
0363-6143(200108)281:2<C407:FDPOAI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
GASTRIC PARIETAL-CELLS; DISRUPT MICROFILAMENT ORGANIZATION; LATRUNCULIN-A; OXYNTIC CELLS; ULTRASTRUCTURAL-CHANGES; RECYCLING HYPOTHESIS; DENDRITIC SPINES; HCL SECRETION; RECRUITMENT; INHIBITION;
Keywords:
parietal cell; latrunculin B; secretion; cytoskeleton;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Forte, JG Univ Calif Berkeley, Dept Mol & Cell Biol, Rm 241 LSA, Berkeley,CA 94720 USA Univ Calif Berkeley Rm 241 LSA Berkeley CA USA 94720 A 94720 USA
Citazione:
D.A. Ammar et al., "Functionally distinct pools of actin in secretory cells", AM J P-CELL, 281(2), 2001, pp. C407-C417

Abstract

Acid secretion by the gastric parietal cell is controlled through movementof vesicles containing the proton pump, the H+-K+-ATPase (HK). We have used latrunculin B (Lat B), which binds to monomeric actin, to investigate actin turnover in the stimulated parietal cell. In isolated gastric glands, relatively high concentrations of Lat B were required to inhibit acid accumulation (ED50 similar to 70 muM). Cultured parietal cells stimulated in the presence of low Lat B (0.1-1 muM) have reduced lamellipodia formation and some aberrant punctate phalloidin-stained structures, but translocation of HKand vacuolar swelling appeared unaffected. High Lat B (10-50 muM) resultedin gross changes in actin organization (punctate phalloidin-stained structures throughout the cell and nucleus) and reduced translocation of HK and vacuolar swelling. Resting parietal cells treated with high Lat B showed minor effects on morphology and F-actin staining. If resting cells treated with high Lat B were washed immediately before stimulation, they exhibited a normal stimulated morphology. These data suggest distinct pools of parietal cell actin: a pool highly susceptible to Lat B primarily involved in motilefunction of cultured cells; and a Lat B-resistant pool, most likely microvillar filaments, that is essential for secretion. Furthermore, the stimulation process appears to accentuate the effects of Lat B, most likely throughLat B binding to monomer actin liberated by the turnover of the motile actin filament pool.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 16:24:34