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Titolo:
Early development of the gubernaculum and cremaster sac in estrogen receptor knockout mice
Autore:
Bartlett, JE; Washburn, T; Eddy, EM; Korach, KS; Temelcos, C; Hutson, JM;
Indirizzi:
Royal Childrens Hosp, Dept Gen Surg, Parkville, Vic 3052, Australia Royal Childrens Hosp Parkville Vic Australia 3052 le, Vic 3052, Australia Royal Childrens Hosp, Res Inst, F Douglas Stephens Surg Res Lab, Melbourne, Vic, Australia Royal Childrens Hosp Melbourne Vic Australia , Melbourne, Vic, Australia NIEHS, Reprod & Dev Toxicol Lab, NIH, Res Triangle Pk, NC 27709 USA NIEHSRes Triangle Pk NC USA 27709 ab, NIH, Res Triangle Pk, NC 27709 USA
Titolo Testata:
UROLOGICAL RESEARCH
fascicolo: 3, volume: 29, anno: 2001,
pagine: 163 - 167
SICI:
0300-5623(200106)29:3<163:EDOTGA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
TESTICULAR DESCENT; REPRODUCTIVE TRACTS; GENE DISRUPTION; FETAL MICE; ER-ALPHA; MOUSE; BETA; CLONING;
Keywords:
ERKO; estrogen receptor alpha; gubernacular development; cremaster muscle; cremaster sac;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Hutson, JM Royal Childrens Hosp, Dept Gen Surg, Parkville, Vic 3052, Australia Royal Childrens Hosp Parkville Vic Australia 3052 2, Australia
Citazione:
J.E. Bartlett et al., "Early development of the gubernaculum and cremaster sac in estrogen receptor knockout mice", UROL RES, 29(3), 2001, pp. 163-167

Abstract

AIM exogenous estrogen causes gubernacular atrophy and cryptorchidism in fetal rodents. Mice with an estrogen receptor-alpha (ER alpha) disrupted gene mutation (alpha ERKO) were studied to determine whether ablation of endogenous estrogen action, through ER alpha, had an effect on gubernacular development. Serial sagittal sections were made of the pelvis in fetal and day 7 postnatal wild-type and alpha ERKO mice with the estrogen receptor-alpha "knockout" gene mutation. Wild-type (n = 24), heterozygote (n = 13) and alpha ERKO mice (n = 12) were sacrificed at 16, 17 and 18 days fetal life and at 7 days postnatally. The size of the gubernaculum, cremaster muscle, cremaster sac, and the width of the sac at both ends in day 7 mice were quantitated by computer analysis. Visually and statistically the ERKO mice could not be separated from the wild-type mice during fetal life. At day 7 postnatally, a thicker cremaster sac was noted morphologically, and also a statistically significant difference was seen in the width of the cremaster sac atthe sac's tip. Sac area, cremaster muscle area and the width of the sac atthe sac's end did not differ significantly. Overall there is minimal phenotypic change observed in the alpha ERKO mouse compared to wild-type at the early developmental stages investigated. However, at postnatal day 7, thereis a difference in the width of the cremasteric sac tip. This suggests that the effect of ER alpha, and thus signaling on the developing gubernaculum, occurs late in development. Alternatively, an action from the recently discovered ER beta may be involved. Exploration of a beta ERKO and the doubleknock-out alpha ERKO/beta ERKO mouse should be informative in evaluating the effect of endogenous estrogens in gubernacular development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:39:31