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Titolo:
Soluble Fas and Fas-ligand in bladder cancer in vitro and in vivo
Autore:
Perabo, FGE; Mattes, RH; Wirger, A; Steiner, G; Kamp, S; Schmidt, D; Pegelow, K; von Ruecker, A; Muller, SC;
Indirizzi:
Univ Bonn, Dept Urol, D-53105 Bonn, Germany Univ Bonn Bonn Germany D-53105 iv Bonn, Dept Urol, D-53105 Bonn, Germany Univ Hosp Ulm, Dept Urol, D-89075 Ulm, Germany Univ Hosp Ulm Ulm GermanyD-89075 p Ulm, Dept Urol, D-89075 Ulm, Germany Med Univ Lubeck, Dept Urol, D-23938 Lubeck, Germany Med Univ Lubeck Lubeck Germany D-23938 ept Urol, D-23938 Lubeck, Germany Univ Bonn, Inst Clin Biochem, D-53105 Bonn, Germany Univ Bonn Bonn Germany D-53105 Inst Clin Biochem, D-53105 Bonn, Germany
Titolo Testata:
UROLOGIC ONCOLOGY
fascicolo: 4, volume: 6, anno: 2001,
pagine: 163 - 169
SICI:
1078-1439(200107/08)6:4<163:SFAFIB>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-MEDIATED CYTOTOXICITY; EXPRESS FAS; IN-VIVO; APOPTOSIS; FORM; SERUM; MECHANISMS; RECEPTORS; MOLECULE; CLONING;
Keywords:
soluble Fas; Fas-ligand; bladder cancer; apoptosis; immune escape;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Perabo, FGE Univ Bonn, Dept Urol, Sigmund Freud Str 25, D-53105 Bonn, Germany Univ Bonn Sigmund Freud Str 25 Bonn Germany D-53105 , Germany
Citazione:
F.G.E. Perabo et al., "Soluble Fas and Fas-ligand in bladder cancer in vitro and in vivo", UROL ONCOL, 6(4), 2001, pp. 163-169

Abstract

Circulating soluble Fas (sFas) and expression of Fas-ligand on cancer cells are mechanisms of immune escape. The aim of the present study was to investigate expression and production of Fas and Fas-Ligand on bladder cancer cell lines of different grade as a basic mechanism of their secretion in vivo. sFas and sFas-ligand serum levels of patients with different stage of bladder cancer were examined to determine the possible clinical use of these molecules as tumor markers. Bladder cancer cell lints RT4 (G1). RT112 (G1),T24 (G3) and SUP (G4) were analyzed by flowcytometry for Fas and Fas-ligand expression. To determine if the fas-ligand gene is transcribed in these bladder cancer cell lines, RT-PCR was performed on mRNA extracted from thesecell lines. Production of sFas and sFas-ligand was examined in cell culture supernatants of the cancer cells as well as in the serum of 62 patients with bladder cancer by a specific ELISA test. We demonstrate that Fas is expressed in similar levels on all human bladder carcinoma cell lines. In T24 (G3) and SUP (G4) cell lines we were able to detect the Fas-ligand protein,whereas no Fas-ligand protein could be found in RT4 and RT112 (G1) cells. Fas-ligand mRNA was expressed in all bladder cancer cell lines. Furthermore, all bladder cancer cell lines produce sFas but no sFas-ligand in spite ofmRNA expression. The range of sFas levels in the serum of all patients with bladder cancer was large and did not show a correlation to the histopathological stage of bladder cancer. Although there is in vitro evidence that sFas and Fas-ligand play a role in bladder cancer, no correlation between the sFas and sFas-ligand serum levels and the histopathological stage of bladder cancer could be found. Therefore, serum sFas and sFas-ligand have to date limited clinical relevance. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 14:04:26