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Titolo:
Demonstration of reinfection and reactivation in HIV-negative autopsied cases of secondary tuberculosis: multilesional genotyping of Mycobacterium tuberculosis utilizing IS6110 and other repetitive element-based DNA fingerprinting
Autore:
du Plessis, DG; Warren, R; Richardson, M; Joubert, JJ; van Helden, PD;
Indirizzi:
Univ Stellenbosch, Fac Med, Dept Anat Pathol, ZA-7505 Tygerberg, South Africa Univ Stellenbosch Tygerberg South Africa ZA-7505 Tygerberg, South Africa Univ Stellenbosch, Fac Med, Dept Med Microbiol, ZA-7505 Tygerberg, South Africa Univ Stellenbosch Tygerberg South Africa ZA-7505 Tygerberg, South Africa Univ Stellenbosch, Fac Med, MRC, Ctr Mol & Cellular Biol,Dept Med Biochem,ZA-7505 Tygerberg, South Africa Univ Stellenbosch Tygerberg South Africa ZA-7505 Tygerberg, South Africa
Titolo Testata:
TUBERCULOSIS
fascicolo: 3, volume: 81, anno: 2001,
pagine: 211 - 220
SICI:
1472-9792(2001)81:3<211:DORARI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXOGENOUS REINFECTION; STRAIN; EPIDEMIOLOGY; INFECTION; TRANSMISSION; PATHOGENESIS; COMMUNITY; PATTERNS; PATIENT; MARKERS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: du Plessis, DG Walton Ctr Neurol & Neurosurg, Dept Neuropathol, Lower Lane, Liverpool L9 7LJ, Merseyside, England Walton Ctr Neurol & Neurosurg LowerLane Liverpool Merseyside England L9 7LJ
Citazione:
D.G. du Plessis et al., "Demonstration of reinfection and reactivation in HIV-negative autopsied cases of secondary tuberculosis: multilesional genotyping of Mycobacterium tuberculosis utilizing IS6110 and other repetitive element-based DNA fingerprinting", TUBERCULOSI, 81(3), 2001, pp. 211-220

Abstract

Setting Secondary tuberculosis may follow reinfection or endogenous reactivation. The design of effective preventive and treatment protocols requiresknowledge about the relative importance of these mechanisms in specific communities. Molecular typing of Mycobacterium tuberculosis has permitted linkage of cases and demonstration of patterns of inter- or intrapatient strain diversity correlating with reinfection and reactivation. Objective: The use of DNA fingerprinting to examine intrapatient strain diversity in autopsied, HIV-negative individuals resident in a high incidencecommunity. Design: Autopsy (12 cases) and pneumonectomy (one case) permitted multilesional sampling for bacterial culture and comparative genotyping. Mycobacterium tuberculosis strains were typed using probes directed against IS6110, the direct repeat sequence (DR) and MTB484(1). Results: In two patients, the demonstration of pulmonary infection by two distinct strains suggested dual infection of these individuals. In one other case, the strain isolated from a Ghon-focus was identical to that obtained from all secondary cavitating lesions, which suggested reactivation of the primary infection. In the remaining cases, all isolates were identical, but primary lesions could not be identified with certainty or were culture negative. One of these cases showed evidence of strain evolution. Conclusions: Multilesional strain genotyping suggested both reinfection and reactivation in a series of HIV-negative autopsied patients with secondary tuberculosis. In most (11/13) cases, the same strain (or clonal variant) was present in all lesions, pulmonary and extrapulmonary. (C) 2001 HarcourtPublishers Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/02/20 alle ore 05:02:11