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Titolo:
Position and sequence requirements for poly(A) length regulation by the poly(A) limiting element
Autore:
Das Gupta, J; Gu, HD; Schoenberg, DR;
Indirizzi:
Ohio State Univ, Dept Mol & Cellular Biochem, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 lar Biochem, Columbus, OH 43210 USA Ohio State Univ, Ohio State Biochem Program, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 hem Program, Columbus, OH 43210 USA Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 rehens Canc, Columbus, OH 43210 USA
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 7, volume: 7, anno: 2001,
pagine: 1034 - 1042
SICI:
1355-8382(200107)7:7<1034:PASRFP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; XENOPUS-LAEVIS; CYTOPLASMIC POLYADENYLATION; CARBOXYL-TERMINUS; BINDING PROTEIN; LAST-INTRON; CIS-ELEMENT; POLYMERASE; SIGNAL; DEFINITION;
Keywords:
3 ' processing; polyadenylation; poly(A)-limiting element; pre-mRNA processing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Schoenberg, DR Ohio State Univ, Dept Mol & Cellular Biochem, 333 Hamilton Hall,1645 Neil Ave, Columbus, OH 43210 USA Ohio State Univ 333 Hamilton Hall,1645 Neil Ave Columbus OH USA 43210
Citazione:
J. Das Gupta et al., "Position and sequence requirements for poly(A) length regulation by the poly(A) limiting element", RNA, 7(7), 2001, pp. 1034-1042

Abstract

The poly(A)-limiting element (PLE) is a cis-acting sequence that acts to limit poly(A) tail length on pre-mRNA to <20 nt, Functional PLEs are presentin a number of genes, underscoring the generality of this control mechanism. The current study sought to define further the position requirements forpoly(A) length regulation and the core sequence that comprises a PLE, Increasing the spacing between the PLE and the upstream 3' splice site or between the PLE and the downstream AAUAAA had no effect on poly(A) length control. However, moving the PLE from the terminal exon to either an upstream exon or intron eliminated poly(A) length control. Poly(A) length control was further evaluated using a battery of constructs in which the PLE was maintained in the terminal exon, but where upstream introns were either deleted, modified, or replaced with a polypyrimidine tract. Poly(A) length control was retained in all cases, indicating that the key feature is the presence ofthe PLE in the terminal exon, A battery of mutations demonstrated the importance of the 5' pyrimidine-rich portion of the element. Finally, UV crosslinking experiments identified an <similar to>62-kDa protein in Hela nuclearextract that binds to a wild-type 23-nt PLE RNA oligonucleotides but not to a mutated nonfunctional form of the element.

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Documento generato il 30/11/20 alle ore 05:47:09