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Titolo:
Specific sequence elements in the 5 ' untranslated regions of rbcL and atpB gene mRNAs stabilize transcripts in the chloroplast of Chlamydomonas reinhardtii
Autore:
Anthonisen, IL; Salvador, ML; Klein, U;
Indirizzi:
Univ Oslo, Dept Biol, N-0316 Oslo, Norway Univ Oslo Oslo Norway N-0316Univ Oslo, Dept Biol, N-0316 Oslo, Norway Univ Valencia, Dept Biochem & Mol Biol, E-46100 Valencia, Spain Univ Valencia Valencia Spain E-46100 & Mol Biol, E-46100 Valencia, Spain
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 7, volume: 7, anno: 2001,
pagine: 1024 - 1033
SICI:
1355-8382(200107)7:7<1024:SSEIT5>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA STABILITY; IN-VIVO; 5'-UNTRANSLATED REGIONS; ESCHERICHIA-COLI; DEGRADATION; EXPRESSION; DNA; POLYADENYLATION; TRANSFORMATION; DETERMINANTS;
Keywords:
5 ' leader sequence; chloroplast gene transcripts; RNA longevity; RNA secondary structure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Klein, U Univ Oslo, Dept Biol, Moltke Moes Vei 32, N-0316 Oslo, Norway Univ Oslo Moltke Moes Vei 32 Oslo Norway N-0316 316 Oslo, Norway
Citazione:
I.L. Anthonisen et al., "Specific sequence elements in the 5 ' untranslated regions of rbcL and atpB gene mRNAs stabilize transcripts in the chloroplast of Chlamydomonas reinhardtii", RNA, 7(7), 2001, pp. 1024-1033

Abstract

Using a series of point mutations in chimeric reporter gene constructs consisting of the 5' regions of the Chlamydomonas chloroplast rbcL or atpB genes fused 5' to the coding sequence of the bacterial uidA (GUS) gene, RNA-stabilizing sequence elements were identified in vivo in the 5' untranslated regions (5' UTRs) of transcripts of the chloroplast genes rbcL and atpB in Chlamydomonas reinhardtii, In chimeric rbcL 5' UTR:GUS transcripts, replacement of single nucleotides in the 10-nt sequence 5'-AUUUCCGGAC-3', extending from positions +38 to +47 relative to the transcripts' 5' terminus, shortened transcript longevity and led to a reduction in transcript abundance ofmore than 95%. A similar mutational analysis of atpB 5' UTR:GUS transcripts showed that the 12-nt atpB 5' UTR sequence 5'-AUAAGCGUUAGU-3', extending from position +31 to position +42, is important for transcript stability and transcript accumulation in the chloroplast of Chlamydomonas. We discuss how the 5' UTR sequence elements, which are predicted to be part of RNA secondary structures, might function in RNA stabilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:22:18