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Titolo:
Effect of fluoxetine on intracellular Ca2+ levels in bladder female transitional carcinoma (BFTC) cells
Autore:
Tang, KY; Lu, T; Chang, CH; Lo, YK; Cheng, JS; Wang, JL; Chang, HT; Jan, CR;
Indirizzi:
Vet Gen Hosp Kaohsiung, Dept Med Educ & Res, Kaohsiung 813, Taiwan Vet GenHosp Kaohsiung Kaohsiung Taiwan 813 & Res, Kaohsiung 813, Taiwan Kaohsiung Vet Gen Hosp, Dept Psychiat, Kaohsiung 813, Taiwan Kaohsiung VetGen Hosp Kaohsiung Taiwan 813 chiat, Kaohsiung 813, Taiwan Kaohsiung Vet Gen Hosp, Dept Internal Med, Kaohsiung 813, Taiwan KaohsiungVet Gen Hosp Kaohsiung Taiwan 813 l Med, Kaohsiung 813, Taiwan Kaohsiung Vet Gen Hosp, Dept Rehabil, Kaohsiung 813, Taiwan Kaohsiung Vet Gen Hosp Kaohsiung Taiwan 813 habil, Kaohsiung 813, Taiwan Kaohsiung Vet Gen Hosp, Dept Surg, Kaohsiung 813, Taiwan Kaohsiung Vet GenHosp Kaohsiung Taiwan 813 Surg, Kaohsiung 813, Taiwan Natl Yang Ming Univ, Sch Med, Taipei 813, Taiwan Natl Yang Ming Univ Taipei Taiwan 813 Univ, Sch Med, Taipei 813, Taiwan Natl Sun Yat Sen Univ, Dept Biol, Kaohsiung 80424, Taiwan Natl Sun Yat SenUniv Kaohsiung Taiwan 80424 ol, Kaohsiung 80424, Taiwan Natl Sun Yat Sen Univ, Inst Life Sci, Kaohsiung 80424, Taiwan Natl Sun YatSen Univ Kaohsiung Taiwan 80424 ci, Kaohsiung 80424, Taiwan
Titolo Testata:
PHARMACOLOGICAL RESEARCH
fascicolo: 5, volume: 43, anno: 2001,
pagine: 503 - 507
SICI:
1043-6618(200105)43:5<503:EOFOIC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEROTONIN UPTAKE INHIBITORS; ANTIDEPRESSANTS; MOBILIZATION; PROZAC;
Keywords:
antidepressant; bladder cancer cells; Ca2+; fluoxetine; fura-2; serotonin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Jan, CR Vet Gen Hosp Kaohsiung, Dept Med Educ & Res, 386 Ta Chung 1st Rd, Kaohsiung 813, Taiwan Vet Gen Hosp Kaohsiung 386 Ta Chung 1st Rd KaohsiungTaiwan 813 n
Citazione:
K.Y. Tang et al., "Effect of fluoxetine on intracellular Ca2+ levels in bladder female transitional carcinoma (BFTC) cells", PHARMAC RES, 43(5), 2001, pp. 503-507

Abstract

The effect of the antidepressant fluoxetine on Ca2+ signaling in cultured cells was largely unknown. The effect of various concentrations of fluoxetine on [Ca2+](i) in populations of bladder female transitional cancer (BFTC)cells was evaluated by using fura-2 as a Ca2+ probe. Fluoxetine increased [Ca2+]i concentration dependently (20-100 muM) with an EC50 value of 30 muM. The response was inhibited by 50-60% on extracellular Ca2+ removal. In Ca2+-free medium, pretreatment with 1 muM thapsigargin tan inhibitor of the endoplasmic reticulum Ca2+ pump) abolished 50 muM fluoxetine-induced Ca2+ release: whereas pretreatment with fluoxetine did not alter the thapsigargin-induced Ca2+ response. Addition of 3 mM Ca2+ increased [Ca2+](i) after pretreatment with 50 muM fluoxetine in Ca2+-free medium, suggestive of fluoxetine-induced capacitative Ca2+ entry. Suppression of inositol 1,4,5-trisphosphate formation by 2 muM U73122 (a phospholipase C inhibitor) did not affect50 muM fluoxetine-induced Ca2+ release. Collectively, this study shows that fluoxetine increased [Ca2+](i) in bladder cancer cells in a concentration-dependent fashion, by releasing Ca2+ from thapsigargin-sensitive Ca2+ stores in an IP3-independent manner, and by inducing Ca2+ influx from extracellular medium. (C) 2001 Academic Press.

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Documento generato il 19/01/20 alle ore 00:28:54