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Titolo:
Sex differences in response to steroids in preterm sheep lungs are not explained by glucocorticoid receptor number or binding affinity
Autore:
Kovar, J; Waddell, BJ; Sly, PD; Willet, KE;
Indirizzi:
Telethon Inst Child Hlth Res, Div Clin Sci, W Perth, WA 6872, Australia Telethon Inst Child Hlth Res W Perth WA Australia 6872 WA 6872, Australia Univ Western Australia, Dept Anat & Human Biol, Perth, WA 6009, Australia Univ Western Australia Perth WA Australia 6009 Perth, WA 6009, Australia
Titolo Testata:
PEDIATRIC PULMONOLOGY
fascicolo: 1, volume: 32, anno: 2001,
pagine: 8 - 13
SICI:
8755-6863(200107)32:1<8:SDIRTS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY-DISTRESS SYNDROME; FETAL LUNG; SURFACTANT PRODUCTION; BETAMETHASONE; LAMBS; RAT;
Keywords:
respiratory distress syndrome; glucocorticoids; glucocorticoid receptors; sexual dimorphism; Scatchard analysis; binding affinity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Kovar, J Telethon Inst Child Hlth Res, Div Clin Sci, POB 855, W Perth, WA 6872, Australia Telethon Inst Child Hlth Res POB 855 W Perth WA Australia 6872 ia
Citazione:
J. Kovar et al., "Sex differences in response to steroids in preterm sheep lungs are not explained by glucocorticoid receptor number or binding affinity", PEDIAT PULM, 32(1), 2001, pp. 8-13

Abstract

We recently reported that prenatal glucocorticoid therapy is less effective at promoting an improvement in lung function in male than in female sheep. This observation, and the higher incidence of respiratory distress syndrome in human males, suggests that the male fetal lung may be less responsiveto glucocorticoids than is the female fetal lung. Since glucocorticoids are known to exert their effects via specific cytoplasmic glucocorticoid receptors (GR), we hypothesized that there may be sexual dimorphism in either the number or binding affinity of lung GR. To test the hypothesis, binding of dexamethasone (a synthetic glucocorticoid, 0.5-40 nM) by cytosolic fractions of male (n = 16) and female (n = 16) fetal sheep lung was measured at 125 days gestation (term = 148 days). Scatchard analysis of dexamethasone binding showed that the total number of GR (Bmax) did not significantly differ between male (346 +/- 42 fmol/mg protein) and female (277 +/- 23 fmol/mg protein) fetuses. The measured binding affinity (Kd) in male fetal lungs (6.85 +/- 0.43 nM) was not significantly different from that in females (8.46 +/- 1.02 nM). In conclusion, this study suggests: that sex differences in fetal sheep lung responses to glucocorticoid therapy are not due to differences in the number or binding affinity of lung GR. (C) Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 09:22:36