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Titolo:
The adapter protein, Grb10, is a positive regulator of vascular endothelial growth factor signaling
Autore:
Giorgetti-Peraldi, S; Murdaca, J; Mas, JC; Van Obberghen, E;
Indirizzi:
Fac Med Nice, IFR 50, INSERM, U145, F-06107 Nice 2, France Fac Med Nice Nice France 2 IFR 50, INSERM, U145, F-06107 Nice 2, France
Titolo Testata:
ONCOGENE
fascicolo: 30, volume: 20, anno: 2001,
pagine: 3959 - 3968
SICI:
0950-9232(20010705)20:30<3959:TAPGIA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSULIN-RECEPTOR SUBSTRATE-1; TYROSINE KINASE; FLK-1/KDR ACTIVATION; PLECKSTRIN HOMOLOGY; BINDING-PROTEIN; NITRIC-OXIDE; SH2 DOMAINS; I RECEPTOR; ANGIOGENESIS; CELLS;
Keywords:
Grb10; VEGF receptor; KDR; angiogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Giorgetti-Peraldi, S Fac Med Nice, IFR 50, INSERM, U145, Ave Valombrose, F-06107 Nice 2, France Fac Med Nice Ave Valombrose Nice France 2 2, France
Citazione:
S. Giorgetti-Peraldi et al., "The adapter protein, Grb10, is a positive regulator of vascular endothelial growth factor signaling", ONCOGENE, 20(30), 2001, pp. 3959-3968

Abstract

Vascular endothelial growth factor (VEGF) is an important regulator of vasculogenesis and angiogenesis. Activation of VEGF receptors leads to the recruitment of SH2 containing proteins which link the receptors to the activation of signaling pathways. Here we report that Grb10, an adapter protein ofwhich the biological role remains unknown, is tyrosine phosphorylated in response to VEGF in endothelial cells (HUVEC) and in 293 cells expressing the VEGF receptor KDR, An intact SH2 domain is required for Grb10 tyrosine phosphorylation in response to VEGF, and this phosphorylation is mediated in part through the activation of Src, In HUVEC, VEGF increases Grb10 mRNA level. Expression of Grb10 in HUVEC or in KDR expressing 293 cells results in an increase in the amount and in the tyrosine phosphorylation of KDR, In 293 cells, this is correlated with the activation of signaling molecules, such as MAP kinase, By expressing mutants of Grb10, we found that the positiveaction of Grb10 is independent of its SH2 domain. Moreover, these Grb10 effects on KDR seem to be specific since Grb10 has no effect on the insulin receptor, and Grb2, another adapter protein, does not mimic the effect of Grb10 on KDR, In conclusion, we propose that VEGF up-regulates Grb10 level, which in turn increases KDR molecules, suggesting that Grb10 could be involved in a positive feedback loop in VEGF signaling.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 12:57:26