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Titolo:
Non invasive quantification of manganese deposits in the rat brain by local measurement of NMR proton T-1 relaxation times
Autore:
Gallez, B; Demeure, R; Baudelet, C; Abdelouahab, N; Beghein, N; Jordan, B; Geurts, M; Roels, HA;
Indirizzi:
Univ Catholique Louvain, Lab Med Chem & Radiopharm, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium Univ Catholique Louvain, Lab Biomed Magnet Resonance, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium Univ Catholique Louvain, Ind Toxicol & Occupat Med Unit, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium Univ Catholique Louvain, Pharmacol Lab, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium
Titolo Testata:
NEUROTOXICOLOGY
fascicolo: 3, volume: 22, anno: 2001,
pagine: 387 - 392
SICI:
0161-813X(200106)22:3<387:NIQOMD>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESONANCE SIGNAL HYPERINTENSITY; CONTRAST AGENTS; LIVER; ENCEPHALOPATHY; ACCUMULATION; INTOXICATION; INJECTION; INTENSITY; MRI;
Keywords:
manganese; neurotoxicity; brain; T-1; MRI; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Gallez, B Univ Catholique Louvain, Lab Med Chem & Radiopharm, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 ssels, Belgium
Citazione:
B. Gallez et al., "Non invasive quantification of manganese deposits in the rat brain by local measurement of NMR proton T-1 relaxation times", NEUROTOXICO, 22(3), 2001, pp. 387-392

Abstract

Up to now, there is no reliable non invasive biomarker for the concentration of manganese (Mn) in the brain after intoxication to this metal. The aimof the present experimental study was to determine the predictive value ofthe localized measurement of the proton NMR relaxation time T-1 as a quantitative estimation of the concentration of Mn in brain. The relationship ofthe proton relaxation rates (1/T-1) was established in rat brain homogenates as a function of the Mn, iron, and copper concentration. Subsequently, an experimental model of Mn neurotoxicity was used: rats were stereotactically injected with increasing amounts of Mn2+ (as MnCl2) in the ventricles. After 3 weeks, local measurements of T-1 were carried out in live rats. Theywere then sacrificed in order to sample the striatum, the cortex and the cerebellum from the brain and to perform a quantitative determination of theconcentration of Mn in these tissues by atomic absorption spectrometry (AAS). The results indicate excellent correlation coefficients between relaxation rates and tissue Mn concentrations (r = 0.84, 0.77 and 0.92 for the striatum, the cortex and the cerebellum. respectively). This methodology offers a unique tool for monitoring the degree of Mn concentration in different areas of the brain in animal models of Mn intoxication. In will be useful for evaluating the efficacy of treatments aimed at decreasing the metal in the brain. The method could be potentially useful for being transposed in the clinical situation for monitoring Mn-exposed workers. (C) 2001 Elsevier Science Inc. All rights reserved

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Documento generato il 12/07/20 alle ore 03:21:23