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Titolo:
Changes in mRNA for ryanodine receptors after transient cerebral ischemia and tolerance induction
Autore:
Dahl, C; Diemer, NH;
Indirizzi:
Univ Copenhagen, Inst Mol Pathol, Neuropathol Lab, DK-1168 Copenhagen, Denmark Univ Copenhagen Copenhagen Denmark DK-1168 , DK-1168 Copenhagen, Denmark
Titolo Testata:
NEUROSCIENCE RESEARCH COMMUNICATIONS
fascicolo: 3, volume: 28, anno: 2001,
pagine: 201 - 210
SICI:
0893-6609(200105/06)28:3<201:CIMFRR>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-RELEASE CHANNEL; HIPPOCAMPAL CA1 NEURONS; GERBIL HIPPOCAMPUS; RAT HIPPOCAMPUS; CA2+ RELEASE; SARCOPLASMIC-RETICULUM; PROTEIN-SYNTHESIS; BRAIN-DAMAGE; RABBIT BRAIN; CELL-DEATH;
Keywords:
ryanodine receptor; ischemia; ischemic tolerance; in situ hybridisation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Dahl, C Univ Copenhagen, Inst Mol Pathol, Neuropathol Lab, DK-1168 Copenhagen, Denmark Univ Copenhagen Copenhagen Denmark DK-1168 8 Copenhagen, Denmark
Citazione:
C. Dahl e N.H. Diemer, "Changes in mRNA for ryanodine receptors after transient cerebral ischemia and tolerance induction", NEUROSC R C, 28(3), 2001, pp. 201-210

Abstract

Disturbance of Ca2+ homeostasis is assumed to precede ischemia-induced neuronal death. Using the 4-vessel occlusion model of cerebral ischemia and induction of ischemic tolerance in the rat, we studied the intracellular Ca2activated Ca2+ channel, the ryanodine receptor (RyR), by means of in situ hybridisation and immunohistochemistry. We found the level of RyR2 mRNA increased in CAI and CA3 of the ischemic vulnerable hippocampus following 3 minutes of tolerance inducing ischemia, whereas 9 minutes of ischemia lead toan increased mRNA level in CA1 in contrast to a decrease in CA3. In ischemic tolerant animals (3 + 8.5 minutes of ischemia) the mRNA level was decreased in CA3. In all experimental groups, RyR1 mRNA remained unaltered, whereas RyR3 mRNA decreased in DG. Immunohistochemistry revealed no alterations of the RyR protein level in all the ischemic groups compared to sham-operated animals. Taken together, these changes do not clarify if the RyR is involved in tolerance induction or in the degenerative process leading to ischemic delayed neuronal death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 01:44:34