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Titolo:
Murine dopamine transporter proximal promoter sequences do not direct lacZtransgene expression to ventral midbrain.
Autore:
Donovan, DM; Nguyen, J; Revay, R; Liu, ZY; Rowley, DL; Barrett, T;
Indirizzi:
NIA, Transgen & Knockout Facil Sect, IRP, NIH, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224 Facil Sect, IRP, NIH, Baltimore, MD 21224 USA NIDA, IRP, NIH, Baltimore, MD 21224 USA NIDA Baltimore MD USA 21224NIDA, IRP, NIH, Baltimore, MD 21224 USA
Titolo Testata:
NEUROSCIENCE RESEARCH COMMUNICATIONS
fascicolo: 3, volume: 28, anno: 2001,
pagine: 151 - 163
SICI:
0893-6609(200105/06)28:3<151:MDTPPS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE-HYDROXYLASE GENE; CENTRAL-NERVOUS-SYSTEM; TISSUE-SPECIFIC EXPRESSION; MESSENGER-RNA EXPRESSION; RAT-BRAIN; 5'-FLANKING REGION; RECEPTOR GENE; SUBUNIT GENE; HIGH-LEVEL; MICE;
Keywords:
dopamine transporter; promoter; lacZ; transgenic; murine; midbrain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Donovan, DM NIA, Transgen & Knockout Facil Sect, IRP, NIH, 5600 Nathan Shock Dr, Baltimore, MD 21224 USA NIA 5600 Nathan Shock Dr Baltimore MD USA 21224 , MD 21224 USA
Citazione:
D.M. Donovan et al., "Murine dopamine transporter proximal promoter sequences do not direct lacZtransgene expression to ventral midbrain.", NEUROSC R C, 28(3), 2001, pp. 151-163

Abstract

Dopamine is an important neurotransmitter involved in a variety of centralnervous system functions including locomotion, cognition and emotions. Thedopamine transporter is uniquely expressed on dopamine neurons, where it'suptake of dopamine constitutes the primary mechanism for terminating dopamine neurotransmission. Although dopamine transporter (DATI) sequences have been isolated from several organisms, little is known about the genomic regulatory elements required for expression. This work describes transgenic analysis of a series of murine DATI promoter fusions to the lacZ reporter gene to localize putative promoter elements. Despite lacZ expression from these constructs in cultured cells, no expression was observed in any dopaminergic loci from either adult or embryonic mouse brains, for any of the four constructs described. Thus, the murine DAT gene sequences sufficient for activating expression are not located within 2.8 kb 5' to the transcription start site, or within the first eight kb of transcribed sequences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 22:26:40