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Titolo:
The polymorphonuclear leukocyte - A new target for erythropoietin
Autore:
Sela, S; Shurtz-Swirski, R; Sharon, R; Manaster, J; Chezar, J; Shkolnik, G; Shapiro, G; Shasha, SM; Merchav, S; Kristal, B;
Indirizzi:
Western Galilee Hosp, Nephrol & Hypertens Unit, IL-22100 Nahariya, Israel Western Galilee Hosp Nahariya Israel IL-22100 IL-22100 Nahariya, Israel Western Galilee Hosp, Eliachar Res Lab, IL-22100 Nahariya, Israel Western Galilee Hosp Nahariya Israel IL-22100 IL-22100 Nahariya, Israel Western Galilee Hosp, Dept Hematol, IL-22100 Nahariya, Israel Western Galilee Hosp Nahariya Israel IL-22100 IL-22100 Nahariya, Israel Technion Israel Inst Technol, Bruce Rappaport Fac Med, IL-31096 Haifa, Israel Technion Israel Inst Technol Haifa Israel IL-31096 L-31096 Haifa, Israel
Titolo Testata:
NEPHRON
fascicolo: 3, volume: 88, anno: 2001,
pagine: 205 - 210
SICI:
0028-2766(200107)88:3<205:TPL-AN>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR MESSENGER-RNA; CENTRAL-NERVOUS-SYSTEM; CELL-LINE; ENDOTHELIAL-CELLS; EXPRESSION; ATHEROSCLEROSIS; IDENTIFICATION; HYPERTENSION; PATHOGENESIS; HEMODIALYSIS;
Keywords:
erythropoietin; erythropoietin receptor; flow cytometry; polymorphonuclear leukocytes; RT-PCR; oxidative stress; inflammation; hemodialysis patients;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Kristal, B Western Galilee Hosp, Nephrol & Hypertens Unit, IL-22100 Nahariya, Israel Western Galilee Hosp Nahariya Israel IL-22100 ahariya, Israel
Citazione:
S. Sela et al., "The polymorphonuclear leukocyte - A new target for erythropoietin", NEPHRON, 88(3), 2001, pp. 205-210

Abstract

A previous study from our laboratory has shown that erythropoietin (EPO), beside its traditional role in erythropoiesis, acts as an alleviator of oxidative stress and inflammation in chronic hemodialysis (HD) patients, conferred in part by activated polymorphonuclear leukocytes (PMNLs), To substantiate this phenomenon, the existence of EPO receptors (EPO-Rs) on PMNL membrane was examined at the transcriptional and translational levels, mRNA for EPO-R was detected in PMNLs using specific primers directed towards the extracellular region of human EPO-R cDNA. The predicted 300-bp fragment was amplified by reverse transcriptase-polymerase chain reaction. Subcloning and sequence analysis revealed 100% homology of this fragment with human EPO-R,The receptor protein was detected on the surface of intact PMNLs using I-125-EpO. Th, protein was further demonstrated by flow cytometric analysis using a fluorescent monoclonal anti-EPO-R. The percentage of PMNLs expressingEPO-R showed a strong correlation with the level of EPO in the serum, suggesting an upregulation of the receptor by the hormone. Taken together with our recent findings that EPO attenuates the oxidative stress and inflammation contributed by PMNLs in HD patients, the detection of functional EPO-R expression in PMNLs places these cells among the nonerythroid, EPO-responsive target populations. Copyright (C) 2001 S. Karger AG. Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 07:45:28