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Titolo:
Molecular cloning and characterization of groESL operon in Streptococcus pneumoniae
Autore:
Kim, SN; Kim, SW; Pyo, SN; Rhee, DK;
Indirizzi:
Sung Kyun Kwan Univ, Coll Pharm, Suwon 440746, South Korea Sung Kyun Kwan Univ Suwon South Korea 440746 , Suwon 440746, South Korea
Titolo Testata:
MOLECULES AND CELLS
fascicolo: 3, volume: 11, anno: 2001,
pagine: 360 - 368
SICI:
1016-8478(20010630)11:3<360:MCACOG>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; BACILLUS-SUBTILIS; DNAK OPERON; STRESS-RESPONSE; ESCHERICHIA-COLI; EXPRESSION; IDENTIFICATION; GENES; TRANSFORMATION; CHAPERONIN;
Keywords:
CIRCE; heat shock protein; groEL; groES; Streptococcus pneumoniae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Rhee, DK Sung Kyun Kwan Univ, Coll Pharm, Suwon 440746, South Korea Sung Kyun Kwan Univ Suwon South Korea 440746 40746, South Korea
Citazione:
S.N. Kim et al., "Molecular cloning and characterization of groESL operon in Streptococcus pneumoniae", MOL CELLS, 11(3), 2001, pp. 360-368

Abstract

GroEL is a major target of the immune defense in infection and seems to benegatively regulated by HrcA in gram-positive organisms. However, HrcA's mechanism has not been elucidated. To elucidate the role of groEL in Streptacoccus pneumoniae, the groESL operon was cloned in Escherichia coli. The promoter region of the pneumococcal groESL operon contained a sigma (A) type promoter and an inverted repeat (CIRCE). A Northern blot analysis of the groEL operon demonstrated that the groESL operon is transcribed as a bf cistronic mRNA, and reached maximum expression 7.5 to 10 min after heat shock. Aprimer extension analysis showed a potential transcription start point at 155 bp upstream of the translation start site, preceding the groES gene. The putative negative regulator of the groEL gene, hrcA, of S. pneumoniae wasrecovered by PCR-based chromosomal walking from grpE locus. A sequence analysis showed a sigma (A) type promoter flanked by 2 CIRCE elements. His-tagged HrcA was overexpressed as a soluble form in E. coil and bound to the CIRCE regions in the promoter of both groESL and dnAK operons in vitro. Additionally, a helix-loop helix motif, a putative DNA binding domain, was foundat the C-terminal of HrcA, These results will help to determine the natureof HrcA in the groESL repression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 01:37:26