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Titolo:
Cigarette smoking and aneuploidy in human sperm
Autore:
Shi, QH; Ko, E; Barclay, L; Hoang, T; Rademaker, A; Martin, R;
Indirizzi:
Alberta Childrens Hosp, Dept Genet, Calgary, AB T2T 5C7, Canada Alberta Childrens Hosp Calgary AB Canada T2T 5C7 gary, AB T2T 5C7, Canada Univ Calgary, Dept Med Genet, Calgary, AB, Canada Univ Calgary Calgary ABCanada gary, Dept Med Genet, Calgary, AB, Canada Northwestern Univ, Sch Med, Chicago, IL USA Northwestern Univ Chicago IL USA hwestern Univ, Sch Med, Chicago, IL USA
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 4, volume: 59, anno: 2001,
pagine: 417 - 421
SICI:
1040-452X(200108)59:4<417:CSAAIH>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; MEN; CHROMOSOME-21; SPERMATOZOA; DISOMY; ABNORMALITIES; REPRODUCTION; DAMAGE; FISH; AGE;
Keywords:
aneuploidy; human sperm; cigarette smoking; fluorescence in situ hybridization; chromosome abnormalities;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Martin, R Alberta Childrens Hosp, Dept Genet, 1820 Richmond Rd SW, Calgary, AB T2T 5C7, Canada Alberta Childrens Hosp 1820 Richmond Rd SW Calgary AB Canada T2T 5C7
Citazione:
Q.H. Shi et al., "Cigarette smoking and aneuploidy in human sperm", MOL REPROD, 59(4), 2001, pp. 417-421

Abstract

Cigarette smoke contains chemicals which are capable of inducing aneuploidy in experimental systems. These chemicals have been shown to reach the male reproductive system, increasing oxidative DNA damage in human sperm and lowering semen quality. We have examined the association between smoking andaneuploid sperm by studying 31 Chinese men with similar demographic characteristics and lifestyle factors except for cigarette smoking. None of the men drank alcohol. These men were divided into three groups: nonsmokers (10 men), light smokers (< 20 cigarettes/day, 11 men), and heavy smokers (greater than or equal to 20 cigarettes/day, 10 men). There were no significant differences in semen parameters or in age across groups. Two multi-color fluorescence in situ hybridizations (FISH) were performed: two-color FISH for chromosomes 13 and 21, and three-color FISH for the sex chromosomes using chromosome 1 as an internal autosomal control for diploidy and lack of hybridization. The mean hybridization efficiency was 99.78%. The frequency of disomy 13 was significantly higher in light and heavy smokers than in nonsmokers, while no significant differences in the frequency of disomy 21, X or Ywere observed across groups. Significant inter-donor heterogeneity in every category of disomic sperm examined was found in both light and heavy smokers, while in nonsmokers only XY disomy showed significant inter-donor differences. Thus, we conclude that cigarette smoking may increase the risk of aneuploidy only for certain chromosomes and that men may have different susceptibilities to aneuploidy in germ cells induced by cigarette smoking. (C)2001 Wiley-Liss, Inc.

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Documento generato il 25/09/20 alle ore 22:53:01