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Titolo:
Cla4 protein kinase is essential for filament formation and invasive growth of Yarrowia lipolytica
Autore:
Szabo, R;
Indirizzi:
Comenius Univ, Fac Nat Sci, Dept Biochem, Bratislava 84215, Slovakia Comenius Univ Bratislava Slovakia 84215 chem, Bratislava 84215, Slovakia
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 1, volume: 265, anno: 2001,
pagine: 172 - 179
SICI:
1617-4615(200103)265:1<172:CPKIEF>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANDIDA-ALBICANS; SACCHAROMYCES-CEREVISIAE; HYPHAL FORMATION; PAK-FAMILY; YEAST; CELL; HOMOLOG; MORPHOGENESIS; STARVATION; INDUCTION;
Keywords:
Yarrowia lipolytica; yeast dimorphism; invasive growth; PAK kinases; cell polarization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Szabo, R Comenius Univ, Fac Nat Sci, Dept Biochem, Bratislava 84215, Slovakia Comenius Univ Bratislava Slovakia 84215 tislava 84215, Slovakia
Citazione:
R. Szabo, "Cla4 protein kinase is essential for filament formation and invasive growth of Yarrowia lipolytica", MOL GENET G, 265(1), 2001, pp. 172-179

Abstract

The non-conventional yeast Yarrowia in lipolytica is a suitable model for the study of yeast dimorphism. In order to identify genes that may be involved in the regulation of this process, random mutagenesis was performed. This led to the isolation of monomorphic mutants that had lost the ability togrow in a hyphal form both in liquid and on solid medium. Filamentation was restored to one of the mutants by transformation with a fragment of Y. lipolytica genomic DNA containing a single 2766-bp ORF. The predicted proteinhas a molecular weight of 99.6 kDa and is highly homologous to the proteinkinases Cla4 of Candida albicans and Saccharomyces cerevisiae, which are members of the p21-activated kinase (PAK) family. Analysis of the putative protein sequence identified conserved C-terminal catalytic, and internal Cdc42p-binding regions, as well as a pleckstrin homology domain typical of PAKkinases. The results indicate that CLA4 is a single-copy gene located on the chromosome V of Y. lipolytica. Deletion of CLA4 is not lethal but completely eliminates the ability to form filaments and to invade agar. A strain lacking a functional CLA4 gene exhibits an aberrant distribution of chitin in the cell wall, indicating a possible role for the Cla4 protein kinase inthe maintenance of cell polarity in Y. lipolytica.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 20:11:36