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Titolo:
Cloning and characterization of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in melon (Cucumis melo L. reticulatus)
Autore:
Kato-Emori, S; Higashi, K; Hosoya, K; Kobayashi, T; Ezura, H;
Indirizzi:
Ibaraki Agr Ctr, Inst Plant Biotechnol, Nishiibaraki 3190292, Japan Ibaraki Agr Ctr Nishiibaraki Japan 3190292 , Nishiibaraki 3190292, Japan Univ Tsukuba, Gene Expt Ctr, Tsukuba, Ibaraki 3058572, Japan Univ TsukubaTsukuba Ibaraki Japan 3058572 sukuba, Ibaraki 3058572, Japan
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 1, volume: 265, anno: 2001,
pagine: 135 - 142
SICI:
1617-4615(200103)265:1<135:CACOTG>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HMG-COA REDUCTASE; A REDUCTASE; ARABIDOPSIS-THALIANA; NUCLEOTIDE-SEQUENCE; HEVEA-BRASILIENSIS; FRUIT-DEVELOPMENT; POTATO-TUBERS; ENZYME; CDNA; BIOSYNTHESIS;
Keywords:
melon; 3-hydroxy-3-methylglutaryl coenzyme A reductase gene; fruit development; yeast complementation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Ezura, H Ibaraki Agr Ctr, Inst Plant Biotechnol, Nishiibaraki 3190292, Japan Ibaraki Agr Ctr Nishiibaraki Japan 3190292 araki 3190292, Japan
Citazione:
S. Kato-Emori et al., "Cloning and characterization of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in melon (Cucumis melo L. reticulatus)", MOL GENET G, 265(1), 2001, pp. 135-142

Abstract

We have isolated a cDNA for Cm-HMGR, encoding 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in melon (Cucumis melo L. reticulatus: Genbank Accession No. AB021862). Cm-HMGR encodes a polypeptide of 588 amino acidsthat contains two transmembrane domains and a catalytic domain. Database searches revealed that Cm-HMGR shows homology to HMG1 (63.7%) and HMG2 (70.3%) of tomato, to HMG1 (77.2%) and HMG2 (69.4%) of Arabidopsis thaliana, andto HMGR of tobacco (72.6%). Functional expression in a HMG-CoA reductase-deficient mutant yeast showed that Cm-HMGR products mediate the synthesis ofmevalonate. Northern analysis revealed that the level of Cm-HMGR mRNA in the fruit increased after pollination and markedly decreased at the end of fruit enlargement. During ripening, Cm-HMGR mRNA levels increased markedly in the fruit. In parallel with mRNA expression, Cm-HMGR activity increased after pollination, whereas no Cm-HMGR activity was detectable during fruit ripening. Our results suggest that Cm-HMGR is important during early post-pollination development of the fruit in melon.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 07:41:20