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Titolo:
Hsp90 levels affect telomere length in yeast
Autore:
Grandin, N; Charbonneau, M;
Indirizzi:
Ecole Normale Super Lyon, UMR CNRS 5665, Yeast Cell Cycle Grp, F-69364 Lyon, France Ecole Normale Super Lyon Lyon France F-69364 e Grp, F-69364 Lyon, France
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 1, volume: 265, anno: 2001,
pagine: 126 - 134
SICI:
1617-4615(200103)265:1<126:HLATLI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRAND BREAKS; DNA-BINDING PROTEIN; SACCHAROMYCES-CEREVISIAE; CATALYTIC SUBUNIT; REVERSE-TRANSCRIPTASE; CDC13 PROTEIN; SIR PROTEINS; RIF PROTEINS; EST GENES; IN-VITRO;
Keywords:
telomere length; Hsc82; Hsp82; STN1; CDC13;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Charbonneau, M Ecole Normale Super Lyon, UMR CNRS 5665, Yeast Cell Cycle Grp, 46 Allee Italie, F-69364 Lyon, France Ecole Normale Super Lyon 46 AlleeItalie Lyon France F-69364
Citazione:
N. Grandin e M. Charbonneau, "Hsp90 levels affect telomere length in yeast", MOL GENET G, 265(1), 2001, pp. 126-134

Abstract

Cdc13 is a Saccharomyces cerevisiae protein that binds to telomeric single-stranded DNA and regulate telomerase activity. Stn1 has been shown by two-hybrid analysis to form a physical complex with Cdc13. Temperature-sensitive mutations in CDC13 and STN1, which are both essential genes, activate a DNA damage-dependent checkpoint which is the cause of the arrest seen in themutant strains. The stn1-13 mutation induces dramatic telomere elongation which is telomerase dependent, as shown here. Additional mutants for STN1, which show a tighter arrest phenotype than stn1-13, were generated in orderto perform genetic screens aiming at uncovering new regulators of telomerase. HSC82, which encodes a conserved molecular chaperone of the Hsp90 family, was thus isolated as a high-dosage suppressor of a temperature-sensitivemutation in STN1. Overexpression of HSC82 also partially suppressed the growth defect of cdc13-1 cells. Overexpression of HSC82 was found to correct the telomeric defect associated with stn1 mutations. Shortening of telomeres was also observed in wild-type cells upon overexpression of HSC82, or of its temperature-inducible homologue, HSP82. These results identify Hsc82/Hsp82 as potential regulators of telomerase in yeast cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:07:14