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Titolo:
Chromosome landing at the bacterial blight resistance gene Xa4 locus usinga deep coverage rice BAC library
Autore:
Wang, W; Zhai, W; Luo, M; Jiang, G; Chen, X; Li, X; Wing, RA; Zhu, L;
Indirizzi:
Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China Chinese AcadSci Beijing Peoples R China 100101 100101, Peoples R China Clemson Univ, Genom Inst, Clemson, SC 29634 USA Clemson Univ Clemson SC USA 29634 Univ, Genom Inst, Clemson, SC 29634 USA
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 1, volume: 265, anno: 2001,
pagine: 118 - 125
SICI:
1617-4615(200103)265:1<118:CLATBB>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-BINDING SITE; XANTHOMONAS-ORYZAE; RFLP MARKERS; DISEASE; CONSTRUCTION; ANALOGS; PCR; IDENTIFICATION; HOMOLOGS; GENOMES;
Keywords:
bacterial artificial chromosome (BAC) library positional cloning; bacterial blight; disease resistance; rice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Zhu, L Chinese Acad Sci, Inst Genet, Bldg 917,Datun Rd, Beijing 100101, Peoples RChina Chinese Acad Sci Bldg 917,Datun Rd Beijing Peoples R China 100101
Citazione:
W. Wang et al., "Chromosome landing at the bacterial blight resistance gene Xa4 locus usinga deep coverage rice BAC library", MOL GENET G, 265(1), 2001, pp. 118-125

Abstract

Xa4 is a dominantly inherited rice gene that confers resistance to Philippine race I of the bacterial blight pathogen Xanthomonas oryzae pv. oryzae in rice. In order to isolate the gene by positional cloning, a bacterial artificial chromosome (BAC) library was constructed from genomic DNA isolated from an Xa4-harboring accession, IRBB56. The library contains 55,296 cloneswith an average insert size of 132 kb, providing 14 rice genome equivalents. Three DNA markers closely linked to Xa4 were used to screen the library. The marker RS13, a resistance gene analogue that co-segregates with Xa4, identified 18 clones, of which four and six, respectively, were simultaneously detected by the other two markers. G181 and L1044. Fingerprinting and Southern analysis indicated that these clones overlapped and define an interval spanning 420 kb. In an F-2 population derived from an indica variety, IR24, and its Xa4-containing near isogenic line (NIL). IRBB4, the susceptibleplants were screened in order to map the Xa4 gene genetically and physically. Out of 24 insert ends isolated from the BACs in the contig, three revealed polymorphisms between IR24 and IRBB4. Two insert ends, 56M22F and 26D24R, flanked Xa4 on each side. Based on the overlap of the BACs, six overlapping clones were considered to include the Xa4 allele, one of which. 106P13,was chosen for further investigation.

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Documento generato il 28/01/20 alle ore 14:54:11