Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Mobility of the piggyBac transposon in embryos of the vectors of Dengue fever (Aedes albopictus) and La Crosse encephalitis (Ae. triseriatus)
Autore:
Lobo, N; Li, X; Hua-Van, A; Fraser, MJ;
Indirizzi:
Univ Notre Dame, Dept Biol, Notre Dame, IN 46556 USA Univ Notre Dame Notre Dame IN USA 46556 pt Biol, Notre Dame, IN 46556 USA
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 1, volume: 265, anno: 2001,
pagine: 66 - 71
SICI:
1617-4615(200103)265:1<66:MOTPTI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-DROSOPHILID INSECTS; YELLOW-FEVER; INTERPLASMID TRANSPOSITION; GERMLINE TRANSFORMATION; PRECISE EXCISION; TRICHOPLUSIA-NI; ELEMENT MARINER; HERMES ELEMENT; UNITED-STATES; MOSQUITO;
Keywords:
Dengue Fever; LaCrosse encephalitis; Aedes; piggyBac; transformation vector;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Fraser, MJ Univ Notre Dame, Dept Biol, Notre Dame, IN 46556 USA Univ NotreDame Notre Dame IN USA 46556 tre Dame, IN 46556 USA
Citazione:
N. Lobo et al., "Mobility of the piggyBac transposon in embryos of the vectors of Dengue fever (Aedes albopictus) and La Crosse encephalitis (Ae. triseriatus)", MOL GENET G, 265(1), 2001, pp. 66-71

Abstract

The re-emergence of arboviral diseases such as Dengue Fever and La Crosse encephalitis is primarily due to the failure of insect vector control strategies. The development of a procedure capable of producing stable germ-linetransformants in the insect vectors of these diseases would bridge the gapbetween gene expression systems being developed to curb vector transmission and the identification of important genes and regulatory sequences and their reintroduction back into the insect genome in the form of vector control strategies. The transposable element piggyBac is capable of transpositionin a variety of insect species. and could serve as a versatile insect transformation vector. Using plasmid-based excision and transposition assays, we report that this short-ITR transposon undergoes precise, transposase-dependent excision and transposition in embryos of Aedes albopictus and Aedes triseriatus, the vectors of Dengue fever and LaCrosse encephalitis, respectively. These assays allow us easily and rapidly to confirm and assess the potential utility of piggyBac as a gene transfer tool in a given species, piggyBac is an exceptionally mobile and versatile genetic transformation vector, comparable to other transposons currently in use for the transformation of insects. The mobility of the piggyBac element seen in both Ae. albopictus and AE. triseriatus is further evidence that it can be employed as a germ-line vector in important insect disease vectors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 22:59:28