Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Advantages of a P-element construct containing MtnA sequences for the identification of patterning and cell determination genes in Drosophila melanogaster
Autore:
Faucheux, M; Netter, S; Bloyer, S; Moussa, M; Boissonneau, E; Lemeunier, F; Wegnez, M; Theodore, L;
Indirizzi:
Univ Paris 11, CNRS UPRES A 8080, Lab Embryol Mol & Expt Chromatine & Dev,F-91405 Orsay, France Univ Paris 11 Orsay France F-91405 hromatine & Dev,F-91405 Orsay, France Inst Genet Humaine, UPR 1142, Montpellier, France Inst Genet Humaine Montpellier France ne, UPR 1142, Montpellier, France CNRS, Biol & Genet Evolut Lab, Gif Sur Yvette, France CNRS Gif Sur Yvette France l & Genet Evolut Lab, Gif Sur Yvette, France
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 1, volume: 265, anno: 2001,
pagine: 14 - 22
SICI:
1617-4615(200103)265:1<14:AOAPCC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEVELOPMENTALLY-REGULATED GENES; MEDIATED ENHANCER DETECTION; POSITION-EFFECT VARIEGATION; DOSAGE-DEPENDENT MODIFIERS; PROTEIN INTERACTION MOTIF; CHROMOSOME-BINDING-SITES; POLYCOMB-GROUP PROTEINS; DORSAL-VENTRAL AXIS; BITHORAX COMPLEX; METALLOTHIONEIN GENE;
Keywords:
BTB/POZ domain; homeotic transformation; spitz; polyhomeotic;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Theodore, L Univ Paris 11, CNRS UPRES A 8080, Lab Embryol Mol & Expt Chromatine & Dev,Bat 425, F-91405 Orsay, France Univ Paris 11 Bat 425 Orsay France F-91405 1405 Orsay, France
Citazione:
M. Faucheux et al., "Advantages of a P-element construct containing MtnA sequences for the identification of patterning and cell determination genes in Drosophila melanogaster", MOL GENET G, 265(1), 2001, pp. 14-22

Abstract

The P[MTW] transposon carries a functional MtnA (metallothionein) gene anda miniwhite reporter gene. When P[MTW] was transformed into Drosophila, many lines were found to show position-dependent expression patterns of the miniwhite or the MtnA transgene. Identification of several of the target genes indicated that this construct behaves as an enhancer or silencer trap. For instance, expression of at least one reporter transgene was shown to correlate with that of the endogenous gene in the case of insertions in Ultrabithorax, four jointed. and the iroquois complex. The frequency of patterns recovered with P[MTW] is higher than that reported for P[LacW]. suggesting that P[MTW] has unusual properties. The possibility of biased insertion of P[MTW] was assayed by screening a sample of 66 MTW lines for modifiers of the extra sex comb phenotype: caused by a hypomorphic allele of polyhomeotic. Seven modifiers were recovered, which could be ranked in two classes: genes involved in leg morphogenesis (including four-jointed and spitz), and genes of the Polycomb- or trithorax-Group, including trithorax and batman, a new gene which encodes a product with a BTB/POZ domain. Taken together, these results indicate that P[MTW] allows the tagging of patterning and cell determination genes, and thus provides a useful tool for identifying new developmental functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 16:08:19