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Titolo:
Two novel Pao-like retrotransposons (Kamikaze and Yamato) from the silkworm species Bombyx mori and B-mandarina: common structural features of Pao-like elements
Autore:
Abe, H; Ohbayashi, F; Sugasaki, T; Kanehara, M; Terada, T; Shimada, T; Kawai, S; Mita, K; Kanamori, Y; Yamamoto, MT; Oshiki, T;
Indirizzi:
Tokyo Univ Agr & Technol, Fac Agr, Dept Biol Prod, Fuchu, Tokyo 1838509, Japan Tokyo Univ Agr & Technol Fuchu Tokyo Japan 1838509 , Tokyo 1838509, Japan Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 Life Sci, Bunkyo Ku, Tokyo 1138657, Japan Natl Inst Radiol Sci, Genome Res Grp, Inage Ku, Anagawa, Chiba 2368555, Japan Natl Inst Radiol Sci Anagawa Chiba Japan 2368555 wa, Chiba 2368555, Japan Kyoto Inst Technol, Drosophila Genet Resource Ctr, Sakyo Ku, Kyoto 6068585, Japan Kyoto Inst Technol Kyoto Japan 6068585 r, Sakyo Ku, Kyoto 6068585, Japan
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 2, volume: 265, anno: 2001,
pagine: 375 - 385
SICI:
1617-4615(200104)265:2<375:TNPR(A>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-LTR RETROTRANSPOSON; POLYMORPHIC DNA RAPD; W-CHROMOSOME; DROSOPHILA-MELANOGASTER; NUCLEOTIDE-SEQUENCE; RETROVIRAL PROTEINS; MOLECULAR-STRUCTURE; GENE; GENOME; REGION;
Keywords:
retrotransposon; Pao; Bombyx mori; Bombyx mandarina;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Abe, H Tokyo Univ Agr & Technol, Fac Agr, Dept Biol Prod, Saiwai Cho 3-5-8, Fuchu, Tokyo 1838509, Japan Tokyo Univ Agr & Technol Saiwai Cho 3-5-8 Fuchu Tokyo Japan 1838509
Citazione:
H. Abe et al., "Two novel Pao-like retrotransposons (Kamikaze and Yamato) from the silkworm species Bombyx mori and B-mandarina: common structural features of Pao-like elements", MOL GENET G, 265(2), 2001, pp. 375-385

Abstract

To characterize the structural features common to Pao-like retrotransposons, we analyzed two lambda phage clones which contain the Pao-like elements from the silkworm species Bombyx mori and B. mandarina, and copies of Pao itself and ninja of Drosophila simulans, amplified by PCR. We previously identified two randomly amplified polymorphic DNAs (RAPDs), W-Kamikaze and W-Yamato, from B. mori and B. mandarina, which are part of two novel Pao-like retrotransposons, Kamikaze and Yamato, respectively. Complete characterization of these and other elements of this group reported here shows that Pao-like elements have common features that distinguish them from the other groups of LTR-retrotransposons. While the elements of the Ty1-copia group encode only one cysteine and histidine (Cys) motif in their gag-like region, the Pao-like elements specify three Cys motifs. The highly conserved D(35)E motif in the integrase domain of the retrotransposon polyprotein seems to beconserved in Pao-like elements, but the number of amino acid residues between D and E varies and is greater than 35. A comparison of the deduced amino acid sequences of the reverse transcriptase domain revealed that the Pao-like elements are members of neither the Ty1-cepia nor the gypsy-Ty3 groups. Therefore, we confirmed that the long-terminal-repeat (LTR) retrotransposons should be divided into three major groups (or families), namely the Ty1-copia, gypsy-Ty3, and Pao-like groups.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:17:17