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Titolo:
Structural and functional characterization of the lexA gene of Xanthomonascampestris pathovar citri
Autore:
Yang, YC; Yang, MK; Kuo, TT; Tu, J;
Indirizzi:
Acad Sinica, Inst Bot, Nankang 115, Taipei, Taiwan Acad Sinica Nankang Taipei Taiwan 115 st Bot, Nankang 115, Taipei, Taiwan Acad Sinica, Inst Mol Biol, Nankang, Taipei, Taiwan Acad Sinica Nankang Taipei Taiwan Inst Mol Biol, Nankang, Taipei, Taiwan Fu Jen Univ, Dept Biol, Taipei, Taiwan Fu Jen Univ Taipei TaiwanFu Jen Univ, Dept Biol, Taipei, Taiwan Natl Def Med Ctr, Inst Life Sci, Taipei, Taiwan Natl Def Med Ctr Taipei Taiwan f Med Ctr, Inst Life Sci, Taipei, Taiwan
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 2, volume: 265, anno: 2001,
pagine: 316 - 326
SICI:
1617-4615(200104)265:2<316:SAFCOT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; RECA GENE; PV. CITRI; SOS BOX; DNA-REPAIR; PROTEIN; REPRESSOR; MUTANT; IDENTIFICATION; CONSTRUCTION;
Keywords:
Xanthomonas campestris pathovar citri; LexA repressor; LexA cleavage; DNA-binding protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Tu, J Acad Sinica, Inst Bot, Nankang 115, Taipei, Taiwan Acad Sinica Nankang Taipei Taiwan 115 , Nankang 115, Taipei, Taiwan
Citazione:
Y.C. Yang et al., "Structural and functional characterization of the lexA gene of Xanthomonascampestris pathovar citri", MOL GENET G, 265(2), 2001, pp. 316-326

Abstract

The role of the LexA protein and, specifically, its effect on recA expression were analyzed in Xanthomonas campestris pathovar citri (X.c. pv. citri). Overexpression of LexA from X.c. pv. citri, in the plant pathogen, as well as in Escherichia coli, results in increased sensitivity to the DNA-damaging agents mitomycin C and ultraviolet radiation, indicating that the recombinant X.c. pv. citri LexA protein is functional in a different bacterial species. Immunoblot analysis revealed that the overexpressed LexA protein functioned as a repressor of recA expression in X.c. pv. citri, and that the mitomycin C-induced increase in the abundance of RecA was accompanied by specific proteolysis of LexA that required RecA. Although the LexA protein from X.c. pv. citri also blocked the expression of recA in E. coli, the E. coli RecA protein was not able to support the autocatalytic cleavage of LexA from the plant pathogen. The transcription start site of the X.c. pv. citrilexA gene was identified, and the region upstream of this gene was shown to confer responsiveness to mitomycin C on a luciferase reporter gene construct. Electrophoretic mobility-shift assays demonstrated that X.c. pv. citriLexA interacts with the promoter region of X.c. pv. citri lexA, as well aswith those of the recA genes of X.c. pv. citri and E. coli. These results indicate that LexA functions as a repressor of gene expression in X.c. pv. citri just as it does in E. coli.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 13:31:51