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Titolo:
A functional OGG1 homologue from Arabidopsis thaliana
Autore:
Dany, AL; Tissier, A;
Indirizzi:
CEA Cadarache, DSV, DEVM, Lab Radiobiol Vegetale, F-13108 St Paul Durance,France CEA Cadarache St Paul Durance France F-13108 3108 St Paul Durance,France
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 2, volume: 265, anno: 2001,
pagine: 293 - 301
SICI:
1617-4615(200104)265:2<293:AFOHFA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
8-OXOGUANINE DNA GLYCOSYLASE; ESCHERICHIA-COLI; SACCHAROMYCES-CEREVISIAE; FPG PROTEIN; GENE; REPAIR; 8-HYDROXYGUANINE; EXPRESSION; CLONING; DAMAGE;
Keywords:
OGG1 homologue; 7,8-dihydro-8-oxoguanine; DNA repair; Arabidopsis thaliana;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Tissier, A CEA Cadarache, DSV, DEVM, Lab Radiobiol Vegetale, F-13108 St Paul Durance,France CEA Cadarache St Paul Durance France F-13108 l Durance,France
Citazione:
A.L. Dany e A. Tissier, "A functional OGG1 homologue from Arabidopsis thaliana", MOL GENET G, 265(2), 2001, pp. 293-301

Abstract

One of the major mutagenic base lesions in DNA caused by exposure to reactive oxygen species is 7,8-dihydro-8-oxoguanine (8-oxoG). Genes coding for DNA repair enzymes that recognise 8-oxoG have been reported in bacteria, yeast, mammals and plants. The prokaryotic and eukaryotic genes are functionalhomologues but differ in their primary sequence. We have cloned, sequenced, and expressed a new Arabidopsis thaliana cDNA that shows sequence homology to the eukaryotic genes coding for 8-oxoG DNA N-glycosylases (OCG1). The 40.3-kDa enzyme it encodes (AtOGG1) introduces a chain break in a double-stranded oligonucleotide specifically at an 8-oxoG residue. In addition, AtOGG1 can form a Schiff base with 8-oxoG in the presence of NaBH4, suggesting that it is a bifunctional DNA N-glycosylase. Furthermore, expression of AtOGG1 in an Escherichia coli strain that is deficient in the repair of 8-oxoGin DNA suppresses its spontaneous-mutator phenotype. Thus, we have demonstrated that AtOGG1 is not only a structural but also a functional eukaryoticOGG1 homologue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 16:24:52