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Titolo:
Quorum sensing controls the synthesis of virulence factors by modulating rsmA gene expression in Erwinia carotovora subsp carotovora
Autore:
Koiv, V; Mae, A;
Indirizzi:
Univ Tartu, Inst Mol & Cell Biol, Estonian Bioctr, EE-51010 Tartu, EstoniaUniv Tartu Tartu Estonia EE-51010 tonian Bioctr, EE-51010 Tartu, Estonia
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 2, volume: 265, anno: 2001,
pagine: 287 - 292
SICI:
1617-4615(200104)265:2<287:QSCTSO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONTROLS EXTRACELLULAR ENZYME; N-(3-OXOHEXANOYL)-L-HOMOSERINE LACTONE; ESCHERICHIA-COLI; GLOBAL CONTROL; SIGNAL; IDENTIFICATION; AUTOINDUCER; REGULATOR;
Keywords:
Erwinia; quorum sensing; RsmA; virulence factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Mae, A Univ Tartu, Inst Mol & Cell Biol, Estonian Bioctr, Riia 23, EE-51010 Tartu, Estonia Univ Tartu Riia 23 Tartu Estonia EE-51010 EE-51010 Tartu, Estonia
Citazione:
V. Koiv e A. Mae, "Quorum sensing controls the synthesis of virulence factors by modulating rsmA gene expression in Erwinia carotovora subsp carotovora", MOL GENET G, 265(2), 2001, pp. 287-292

Abstract

The plant-pathogenic bacterium Erwinia carotovora subsp. carotovora (Ecc) causes disease mainly by means of a number of extracellular plant cell wall-degrading enzymes (PCWDEs), also referred to as virulence factors. The production of PCWDEs is coordinately activated by the diffusible signal molecule N-acyl-homoserine lactone (HSL) in a population density-dependent manner("quorum sensing"). ExpI is the enzyme responsible for the synthesis of HSL. The Rsm system negatively regulates the production of PCWDEs. It includes three components: RsmA is an RNA-binding protein which promotes mRNA decay; rsmB is a unique regulator RNA, and RsmC regulates expression of rsmA positively and of rsmB negatively. We report here that in an expI knockout mutant of Ece strain SCC3193, the levels of rsmA and rsmB RNA are remarkably enhanced in comparison to the wild-type strain, while the level of the rsmCtranscript is not affected. The increase in transcription of rsmA in the expI strain represses production of PCWDEs, which in turn leads to the avirulent phenotype of this mutant. In the expI(-) mutant, addition of exogenousHSL caused repression of rsmA and rsmB transcription to the wildtype level, whereas the expression of rsmC was not affected. Taken together, these data suggest that HSL affects the expression of rsmA, and that this effect isnot mediated by RsmC. This specific effect and the previous demonstration that HSL is required for PCWDE production in Ece support the hypothesis that regulation by quorum sensing in Ecc, in contrast to most other systems already described, requires HSL to repress rsmA transcription, which in turn leads to the activation of PCWDE production. A model is presented that explains how HSL controls the production of PCWDEs by modulating the expressionof rsmA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:32:03