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Titolo:
The IML3/MCM19 gene of Saccharomyces cerevisiae is required for a kinetochore-related process during chromosome segregation
Autore:
Ghosh, SK; Poddar, A; Hajra, S; Sanyal, K; Sinha, P;
Indirizzi:
Bose Inst, Dept Biochem, Calcutta 700054, W Bengal, India Bose Inst Calcutta W Bengal India 700054 Calcutta 700054, W Bengal, India
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 2, volume: 265, anno: 2001,
pagine: 249 - 257
SICI:
1617-4615(200104)265:2<249:TIGOSC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
YEAST SACCHAROMYCES; BUDDING YEAST; CELL-CYCLE; CENTROMERE PROTEIN; MITOTIC SPINDLE; S-CEREVISIAE; TRANSMISSION; MUTANTS; MINICHROMOSOMES; IDENTIFICATION;
Keywords:
iml; mcm; chromosome segregation; kinetochores; yeast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Sinha, P Bose Inst, Dept Biochem, P1-12 CIT Scheme 7 M, Calcutta 700054, WBengal, India Bose Inst P1-12 CIT Scheme 7 M Calcutta W Bengal India 700054 dia
Citazione:
S.K. Ghosh et al., "The IML3/MCM19 gene of Saccharomyces cerevisiae is required for a kinetochore-related process during chromosome segregation", MOL GENET G, 265(2), 2001, pp. 249-257

Abstract

The mcm19 mutation in budding yeast affects minichromosome maintenance. Inthis work we have shown that this mutation leads to defects in the segregation of minichromosomes and chromosomes. The mutant cells show defective kinetochore function as judged by three criteria - relaxation of the transcriptional block normally associated with a CEN box, stable maintenance of a dicentric plasmid in mutant cells, and mild sensitivity to the antimicrotubule drug benomyl. The MCM19 gene has been cloned and found to be the same asIML3, which codes for the ORF YBR107C. Deletion of the gene was not lethal, nor did it confer any growth defects on the mutant cells. However, the mcm19 null mutation conferred growth defects in the presence of a mutation inthe TUB1 gene coding for alpha-tubulin. Two-hybrid experiments showed an interaction between Im13p/Mcm19p and the kinetochore protein Ch14, indicating that the Im13/Mcm19 protein has a role in kinetochore function.

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Documento generato il 04/07/20 alle ore 03:38:06