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Titolo:
Suppression of gamma ray-induced illegitimate recombination in Escherichiacoli by the DNA binding protein H-NS
Autore:
Shanado, Y; Hanada, K; Ikeda, H;
Indirizzi:
Kitasato Inst, Ctr Basic Res, Minato Ku, Tokyo 1088642, Japan Kitasato Inst Tokyo Japan 1088642 c Res, Minato Ku, Tokyo 1088642, Japan Univ Tokyo, Inst Med Sci, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 Tokyo, Inst Med Sci, Tokyo 1088639, Japan
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 2, volume: 265, anno: 2001,
pagine: 242 - 248
SICI:
1617-4615(200104)265:2<242:SOGRIR>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIO TRANSDUCING PHAGE; HISTONE-LIKE PROTEINS; MAMMALIAN-CELLS; CURVED DNA; BENT DNA; IONIZING-RADIATION; DELETIONS; HELICASE; HOTSPOT; GENOME;
Keywords:
ionizing radiation; nonhomologous end-joining; bent DNA; kin17 protein; short homology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Ikeda, H Univ Tokyo, Inst Med Sci, PO Takanawa, Tokyo 1088639, Japan Univ Tokyo PO Takanawa Tokyo Japan 1088639 Tokyo 1088639, Japan
Citazione:
Y. Shanado et al., "Suppression of gamma ray-induced illegitimate recombination in Escherichiacoli by the DNA binding protein H-NS", MOL GENET G, 265(2), 2001, pp. 242-248

Abstract

To study the mechanism of gamma -ray-induced illegitimate recombination, we examined the formation of lambda bio transducing phage in Escherichia coli after gamma -ray irradiation. We show that gamma -ray irradiation enhances the formation of lambda bio transducing phage during prophage induction. Moreover, an hns mutation synergistically enhanced the incidence of gamma -ray-induced illegitimate recombination. Next we determined the sequences atthe recombination junctions of the lambda bio transduclllg phages induced by gamma -ray irradiation. Most of the recombination sites coincided with known hotspots. Among them, hotspot I accounted for 67% and 77% of gamma -ray-induced lambda bio transducing phages: in the wild type and the hns mutant, respectively. Therefore, the recombination sites appear to occur mostly at hotspot I or at other hotspots, but rarely at non-hotspot sites. These results suggest that types of DNA damage other than the double-strand breaksinduced at random sites are mainly responsible for the introduction of thesite-specific or region-specific DNA double strand breaks that lead to recombination at the hotspots. The results also showed that the recombination events took place between DNA sequences possessing short stretches of homology. H-NS protein, which binds to curved DNA, suppresses illegitimate recombination:ln: the presence and absence of gamma -ray irradiation. Models forgamma -ray-induced illegitimate recombination are discussed.

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Documento generato il 06/04/20 alle ore 01:35:04