Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Functional expression and characterization of a voltage-gated Ca(v)1,3 (alpha(1D)) calcium channel subunit from an insulin-secreting cell line
Autore:
Scholze, A; Plant, TD; Dolphin, AC; Nurnberg, B;
Indirizzi:
Univ Ulm, Abt Pharmakol & Toxikol, D-89081 Ulm, Germany Univ Ulm Ulm Germany D-89081 t Pharmakol & Toxikol, D-89081 Ulm, Germany Free Univ Berlin, Inst Pharmakol, D-14195 Berlin, Germany Free Univ Berlin Berlin Germany D-14195 armakol, D-14195 Berlin, Germany Univ London Univ Coll, Dept Pharmacol, London WC1E 6BT, England Univ London Univ Coll London England WC1E 6BT , London WC1E 6BT, England
Titolo Testata:
MOLECULAR ENDOCRINOLOGY
fascicolo: 7, volume: 15, anno: 2001,
pagine: 1211 - 1221
SICI:
0888-8809(200107)15:7<1211:FEACOA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC-BETA-CELLS; PROTEIN-KINASE-C; MU-OPIOID RECEPTOR; CA2+ CHANNELS; T-TYPE; XENOPUS-OOCYTES; B-CELLS; DEPENDENT MODULATION; OMEGA-CONOTOXIN; RAT-BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Nurnberg, B Univ Ulm, Abt Pharmakol & Toxikol, Albert Einstein Allee 11, D-89081 Ulm, Germany Univ Ulm Albert Einstein Allee 11 Ulm Germany D-89081 Germany
Citazione:
A. Scholze et al., "Functional expression and characterization of a voltage-gated Ca(v)1,3 (alpha(1D)) calcium channel subunit from an insulin-secreting cell line", MOL ENDOCR, 15(7), 2001, pp. 1211-1221

Abstract

L-type calcium channels mediate depolarization-induced calcium influx in insulin-secreting cells and are thought to be modulated by G protein-coupledreceptors (GPCRs). The major fraction of L-type alpha (1)-subunits in pancreatic beta -cells is of the neuroendocrine subtype (Ca(v)1.3 or alpha (1D)). Here we studied the biophysical properties and receptor regulation of a Ca(v)1.3 subunit previously cloned from HIT-T15 cells. In doing so, we compared this neuroendocrine Ca(v)1.3 channel with the cardiac L-type channel Ca(v)1.2a (or alpha (1C-a)) after expression together with alpha (2)delta- and beta (3)-subunits in Xenopus oocytes. Both the current voltage relation and voltage dependence of inactivation for the neuroendocrine Ca(v)1.3 channel were shifted to more negative potentials compared with the cardiac Ca(v)1.2 channel. In addition, the Ca(v)1.3 channel activated and inactivated more rapidly than the Ca(v)1.2a channel. Both subtypes showed a similar sensitivity to the dihydropyridine (+)isradipine. More interestingly, the Ca(v)1.3 channels were found to be stimulated by ligand-bound G(1)/G(0)-coupled GPCRs whereas a neuronal Ca(v)2.2 (or alpha (1B) ) channel was inhibited. The observed receptor-induced stimulation of Ca(v)1.3 channels could be mimicked by phorbol-12-myristate-13-acetate and was sensitive to inhibitors of protein kinases, but not to the phosphoinositol-3-kinase-inhibitor wortmannin, pointing to serine/ threonine kinase-dependent regulation. Taken together, we describe a neuroendocrine L-type Ca(v)1.3 calcium channel that is stimulated by G(1)/G(0)-coupled GPCRs and differs significantly in distinct biophysical characteristics from the cardiac subtype (Ca(v)1.2a), suggestingthat the channels have different roles in native cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:27:42