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Titolo:
Using the yeast interaction trap and other two-hybrid-based approaches to study protein-protein interactions
Autore:
Toby, GG; Golemis, EA;
Indirizzi:
Fox Chase Canc Ctr, Div Basic Sci, Philadelphia, PA 19111 USA Fox Chase Canc Ctr Philadelphia PA USA 19111 , Philadelphia, PA 19111 USA Univ Penn, Sch Med, Cell & Mol Biol Grp, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Biol Grp, Philadelphia, PA 19104 USA
Titolo Testata:
METHODS
fascicolo: 3, volume: 24, anno: 2001,
pagine: 201 - 217
SICI:
1046-2023(200107)24:3<201:UTYITA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
REVERSE 2-HYBRID SYSTEM; BETA-GALACTOSIDASE COMPLEMENTATION; HIGH-EFFICIENCY TRANSFORMATION; INTERACTIONS IN-VIVO; TRANSCRIPTIONAL ACTIVATOR; GENETIC SELECTION; FALSE POSITIVES; 3-HYBRID SYSTEM; SACCHAROMYCES-CEREVISIAE; IDENTIFICATION;
Keywords:
two-hybrid; reverse two-hybrid; split two-hybrid; balt; prey; SRS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Golemis, EA Fox Chase Canc Ctr, Div Basic Sci, 7701 Burholme Ave,W406, Philadelphia, PA 19111 USA Fox Chase Canc Ctr 7701 Burholme Ave,W406 Philadelphia PA USA 19111
Citazione:
G.G. Toby e E.A. Golemis, "Using the yeast interaction trap and other two-hybrid-based approaches to study protein-protein interactions", METHODS, 24(3), 2001, pp. 201-217

Abstract

The detection of physical interaction between two or more molecules of interest can be facilitated if the act of association between the interactive partners leads to the production of a readily observed biological or physical readout. Many interacting molecule pairs (X, Y) can be made to induce such a readout if X and Y are each fused to defined protein elements with desired properties. For example, in the yeast forward two-hybrid system, X is synthesized as a translational fusion to a DNA-binding domain (DBD), Y is synthesized as a fusion to a transcriptional activation domain (AD), and coexpression of DBD-X and AD-Y induces transcription of easily scored responsive reporters. Other approaches use paradigms based on the artifical production of two, hybrid, molecules, but substitute a variety of readouts including the repression of transcription, activation of signal transduction pathways, or reconstitution of a disrupted enzymatic activity. In this article, we summarize a number of two-hybrid-based approaches, and detail the use ofthe forward yeast two-hybrid system in a screen to identify novel interacting partners for a protein of interest. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 08:00:07