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Titolo:
Effects of insert size on transposition efficiency of the Sleeping Beauty transposon in mouse cells
Autore:
Karsi, A; Moav, B; Hackett, P; Liu, ZJ;
Indirizzi:
Auburn Univ, Fish Mol Genet & Biotechnol Lab, Dept Fisheries & Allied Aquacultures, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 Allied Aquacultures, Auburn, AL 36849 USA Auburn Univ, Program Cell & Mol Biosci, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 m Cell & Mol Biosci, Auburn, AL 36849 USA Tel Aviv Univ, Fac Life Sci, Dept Zool, IL-69978 Tel Aviv, Israel Tel AvivUniv Tel Aviv Israel IL-69978 t Zool, IL-69978 Tel Aviv, Israel Univ Minnesota, Dept Genet Cell Biol & Dev, St Paul, MN 55108 USA Univ Minnesota St Paul MN USA 55108 ell Biol & Dev, St Paul, MN 55108 USA
Titolo Testata:
MARINE BIOTECHNOLOGY
fascicolo: 3, volume: 3, anno: 2001,
pagine: 241 - 245
SICI:
1436-2228(200105/06)3:3<241:EOISOT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TC1-LIKE TRANSPOSON; P-ELEMENT; ZEBRAFISH; DROSOPHILA; FAMILY; GENE; EVOLUTION; SEQUENCES; FISH;
Keywords:
Tcl-like; integration; gene transfer; transgenic fish;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, ZJ Auburn Univ, Fish Mol Genet & Biotechnol Lab, Dept Fisheries & Allied Aquacultures, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 quacultures, Auburn, AL 36849 USA
Citazione:
A. Karsi et al., "Effects of insert size on transposition efficiency of the Sleeping Beauty transposon in mouse cells", MAR BIOTEC, 3(3), 2001, pp. 241-245

Abstract

Transposon vectors are widely used in prokaryotic and lower eukaryotic systems. However, they were not available for use in vertebrate animals until the recent reconstitution of a synthetic fish transposon, Sleeping Beauty (SB). The reacquisition of transposability of the SE transposase fostered great enthusiasm for using transposon vectors as tools in vertebrate animals,particularly for gene transfer to facilitate accelerated integration of transgenes into chromosomes. Here, we report the effects of insert sizes on transposition efficiency of SE. A significant effect of insert size on efficiency of transposition by SE was found. The SE transposase enhanced the integration efficiency effectively for SE transposon up to approximately 5.6 kb, but lost its ability to enhance the integration efficiency when the transposon size was increased to 9.1 kb. This result indicates that the SE transposon system is highly applicable for transferring small genes, but may not be applicable for transferring very large genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/02/20 alle ore 21:05:52