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Titolo:
Determination of fenticonazole and its impurities by capillary electrophoresis and high performance liquid chromatography
Autore:
Quaglia, MG; Donati, E; Bossu, E; Desideri, N; Campana, F;
Indirizzi:
Univ Rome La Sapienza, Dipartimento Studi Farmaceut, I-00185 Rome, Italy Univ Rome La Sapienza Rome Italy I-00185 Farmaceut, I-00185 Rome, Italy Ist Super Sanita, Lab Chim Farmaco, I-00161 Rome, Italy Ist Super Sanita Rome Italy I-00161 ab Chim Farmaco, I-00161 Rome, Italy Recordati SpA, Campoverde Aprilia, LT, Italy Recordati SpA Campoverde Aprilia LT Italy Campoverde Aprilia, LT, Italy
Titolo Testata:
JOURNAL OF SEPARATION SCIENCE
fascicolo: 5, volume: 24, anno: 2001,
pagine: 392 - 396
SICI:
1615-9314(200105)24:5<392:DOFAII>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMIDAZOLE; SEPARATION; EXTRACTION; MICONAZOLE; MECHANISM; CREAMS; DRUGS;
Keywords:
high performance liquid chromatography; capillary electrophoresis; quality control; fenticonazole;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Quaglia, MG Univ Rome La Sapienza, Dipartimento Studi Farmaceut, Piazzale Aldo Moro 5,I-00185 Rome, Italy Univ Rome La Sapienza Piazzale Aldo Moro 5 Rome Italy I-00185
Citazione:
M.G. Quaglia et al., "Determination of fenticonazole and its impurities by capillary electrophoresis and high performance liquid chromatography", J SEP SCI, 24(5), 2001, pp. 392-396

Abstract

Fenticonazole, a topical antifungal agent containing a stereogenic centre,is used in therapy as a racemic mixture. Five related compounds can be found as impurities in the drug. We propose a HPLC method using as stationary phase a RP-8 column eluted with different gradients of acetonitrile/phosphate buffer (pH 6) for simultaneous determination of the drug and these impurities. We also studied a high performance capillary electrophoresis (HPCE) method for quality control of fenticonazole. The separation of fenticonazole from all its impurities by HPCE was obtained in a relative short capillary (40 cm, effective length 34 cm, 50 mum ID) with a running buffer of 30mM phosphate (pH3) containing 8mM trimethyl-beta -cyclodextrin. Under these experimental conditions very good separation of fenticonazole from each individual impurity is obtained in less than 20 minutes. The choice of cyclodextrin added to the running buffer was dictated by the chiral nature of fenticonazole; trimethyl-beta -cyclodextrin is mainly used as chiral selector. The optimised HPLC and HPCE methods are compared.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 08:57:18