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Titolo:
Elevated mRNA expression for supervillin and vascular endothelial growth factor in human neutrophils stimulated with lipopolysaccharide from Porphyromonas gingivalis
Autore:
Morozumi, T; Kubota, T; Sugita, N; Ohsawa, Y; Yamazaki, K; Yoshie, H;
Indirizzi:
Niigata Univ, Fac Dent, Dept Periodontol, Niigata 9518514, Japan Niigata Univ Niigata Japan 9518514 t Periodontol, Niigata 9518514, Japan
Titolo Testata:
JOURNAL OF PERIODONTAL RESEARCH
fascicolo: 3, volume: 36, anno: 2001,
pagine: 160 - 168
SICI:
0022-3484(200106)36:3<160:EMEFSA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR NECROSIS FACTOR; MESSENGER-RNA; POLYMORPHONUCLEAR LEUKOCYTES; PERIODONTAL-DISEASE; TISSUE INHIBITORS; MOLECULAR-CLONING; ESCHERICHIA-COLI; CREVICULAR FLUID; BINDING-PROTEIN; KAPPA-B;
Keywords:
supervillin; vascular endothelial growth factor; neutrophil; Porphyromonas gingivalis-lipopolysaccharide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshie, H Niigata Univ, Fac Dent, Dept Periodontol, 2-5274 Gakkocho, Niigata 9518514, Japan Niigata Univ 2-5274 Gakkocho Niigata Japan 9518514 18514, Japan
Citazione:
T. Morozumi et al., "Elevated mRNA expression for supervillin and vascular endothelial growth factor in human neutrophils stimulated with lipopolysaccharide from Porphyromonas gingivalis", J PERIOD RE, 36(3), 2001, pp. 160-168

Abstract

Differential gene expression was examined in human neutrophils stimulated with lipopolysaccharide front Porphyromonas gingivalis (P. gingivalis-LPS) using RNA fingerprinting by arbitrarily primed polymerase chain reaction (RAP-PCR). LPS from Escherichia coli (E. coli-LPS) was used as control. More than 200 differently expressed transcripts were found in X subjects showingdifferential regulation at the transcriptional level. Densitometric analyses revealed that 42-100 genes were upregulated. while 53 116 genes were downregulated by P. gingivalis-LPS compared with E, coli-LPS. The results of sequencing identification showed the presence of supervillin (SVIL) and vascular endothelial growth factor (VEGF) genes in the clones which were upregulated by P. gingivalis-LPS. Consequently. semiquantitative analyses proved that the level of mRNA for SVIL and VEGF were significantly higher in P. gingivalis-LPS-stimulated neutrophils than in other bacterial(E. coli. Actinobacillus actinomycetemcomitans. Prevotella intermedia) LPS- or synthetic lipid A-stimulated neutrophils. Our findings suggest that an elevated mRNA expression for SVIL could be associated with impaired function of neutrophilswhen stimulated by P. gingivalis-LPS. Further. the VEGF mRNA over-expression might be related to the pathogenesis of P. gingivalis-associated periodontitis. The RAP-PCR technique used in this study enabled us to identify a number of P. gingivalis-LPS regulated genes which hitherto have not been reported.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 17:12:59