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Titolo:
Molecular cloning and characterization of neural activity-related RING finger protein (NARF): a new member of the RBCC family is a candidate for the partner of myosin V
Autore:
Ohkawa, N; Kokura, K; Matsu-ura, T; Obinata, T; Konishi, Y; Tamura, T;
Indirizzi:
Chiba Univ, Fac Sci, Dept Biol, Inage Ku, Chiba 2638522, Japan Chiba UnivChiba Japan 2638522 Dept Biol, Inage Ku, Chiba 2638522, Japan Chiba Univ, Grad Sch Sci & Technol, Mol Biol Lab, Chiba 2638522, Japan Chiba Univ Chiba Japan 2638522 chnol, Mol Biol Lab, Chiba 2638522, Japan Japan Sci & Technol Corp, CREST, Kawaguchi, Japan Japan Sci & Technol Corp Kawaguchi Japan Corp, CREST, Kawaguchi, Japan
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 1, volume: 78, anno: 2001,
pagine: 75 - 87
SICI:
0022-3042(200107)78:1<75:MCACON>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; PENTYLENETETRAZOLE-INDUCED SEIZURES; HIPPOCAMPAL CA1 REGION; IMMEDIATE-EARLY GENE; RAT HIPPOCAMPUS; DENTATE GYRUS; LATE-PHASE; PLASMINOGEN-ACTIVATOR; NEUROTROPHIC FACTOR; PURKINJE-CELLS;
Keywords:
gene expression; hippocampus; neural activity; neural plasticity; pentylenetetrazol; seizure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Ohkawa, N Chiba Univ, Fac Sci, Dept Biol, Inage Ku, 1-33 Yayoi Cho, Chiba 2638522, Japan Chiba Univ 1-33 Yayoi Cho Chiba Japan 2638522 ba 2638522, Japan
Citazione:
N. Ohkawa et al., "Molecular cloning and characterization of neural activity-related RING finger protein (NARF): a new member of the RBCC family is a candidate for the partner of myosin V", J NEUROCHEM, 78(1), 2001, pp. 75-87

Abstract

Activity-dependent synaptic plasticity has been thought to be a cellular basis of memory and learning. The late phase of longterm potentiation (L-LTP), distinct from the early phase, lasts for up to 6 h and requires de novo synthesis of mRNA and protein. Many LTP-related genes are enhanced in the hippocampus during pentyrenetetrazol (PTZ)- and kainate (KA)-mediated neuralactivation: In this study, mice were administered intraperitoneal injections of PTZ 10 times, once every 48 h, and showed an increase in seizure indexes. Genes related to plasticity were efficiently induced in the mouse hippocampus. We used a PCR-based cDNA subtraction method to isolate genes that are expressed in the hippocampus of repeatedly PTZ-treated mice. One of these genes, neural activity-related RING finger protein (NARF), encodes a newprotein containing a RING finger, B-box zinc finger, coiled-coil (RBCC domain) and beta -propeller (NHL) domain, and is predominantly expressed in the brain, especially in the hippocampus. In addition, KA up-regulated the expression of NARF mRNA in the hippocampus. This increase correlated with theactivity of the NMDA receptor. By analysis using GFP-fused NARF, the protein was found to localize in the cytoplasm. Enhanced green fluorescent protein-fused NARF was also localized in the neurites and growth cones in neuronal differentiated P19 cells. The C-terminal beta -propeller domain of NARF interacts with myosin V, which is one of the most abundant myosin isoforms in neurons. The NARF protein increases in hippocampal and cerebellar neurons after PTZ-induced seizure. These observations indicated that NARF expression is enhanced by seizure-related neural activities, and NARF may contribute to the alteration of neural cellular mechanisms along with myosin V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 05:01:34