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Titolo:
Enterovirus sequences resembling coxsackievirus A2 detected in stool and spleen from a girl with fatal myocarditis
Autore:
Bendig, JWA; OBrien, PS; Muir, P; Porter, HJ; Caul, EO;
Indirizzi:
W Pk Hosp, Dept Med Microbiol, PHLS Coxsackievirus Reference Unit, Surrey KT19 8PB, England W Pk Hosp Surrey England KT19 8PB ference Unit, Surrey KT19 8PB, England Univ London Kings Coll, Guys Kings & St Thomas Sch Med, Dept Infect, London WC2R 2LS, England Univ London Kings Coll London England WC2R 2LS London WC2R 2LS, England St Michaels Hosp, Dept Paediat Pathol, Bristol, Avon, England St Michaels Hosp Bristol Avon England iat Pathol, Bristol, Avon, England Bristol Publ Hlth Lab, Bristol, Avon, England Bristol Publ Hlth Lab Bristol Avon England h Lab, Bristol, Avon, England
Titolo Testata:
JOURNAL OF MEDICAL VIROLOGY
fascicolo: 4, volume: 64, anno: 2001,
pagine: 482 - 486
SICI:
0146-6615(200108)64:4<482:ESRCAD>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; RNA; DISEASE; CLASSIFICATION; INFECTION; VP1;
Keywords:
enterovirus PCR; suckling mouse inoculation; heart infection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Bendig, JWA W Pk Hosp, Dept Med Microbiol, PHLS Coxsackievirus Reference Unit, Horton Lane, Surrey KT19 8PB, England W Pk Hosp Horton Lane Surrey England KT19 8PB 19 8PB, England
Citazione:
J.W.A. Bendig et al., "Enterovirus sequences resembling coxsackievirus A2 detected in stool and spleen from a girl with fatal myocarditis", J MED VIROL, 64(4), 2001, pp. 482-486

Abstract

A 10-year-old girl who died suddenly was found at post mortem to have myocarditis. Virus could not be cultured from post-mortem stool, spleen or heart but enterovirus RNA was detected in stool and spleen by PCR, and the stool caused flaccid paralysis in newborn suckling mice. A 654 base pair (bp) sequence from the capsid-coding region of the viral genome was amplified from an affected mouse and sequenced. Using this sequence, strain-specific nested primers were designed and used to amplify viral sequences directly fromstool and spleen. These sequences were identical to each other and to thatobtained from the infected mouse, and most closely resembled Coxsackievirus A2, an uncommon serotype rarely associated with myocarditis. Testing spleen tissue may be useful in etiological investigation of suspected viral myocarditis. PCR proved more sensitive than suckling mouse inoculation in detecting this Coxsackievirus, but a combination of both methods was required for genotypic characterization. J. Med. Virol. 64:482-486, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 23:12:03