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Titolo:
Investigation of tyrosine nitration in proteins by mass spectrometry
Autore:
Petersson, AS; Steen, H; Kalume, DE; Caidahl, K; Roepstorff, P;
Indirizzi:
Univ So Denmark, Dept Biochem & Mol Biol, Odense Univ, DK-5230 Odense M, Denmark Univ So Denmark Odense Denmark M Odense Univ, DK-5230 Odense M, Denmark Sahlgrens Univ Hosp, Dept Clin Physiol, S-41345 Gothenburg, Sweden Sahlgrens Univ Hosp Gothenburg Sweden S-41345 S-41345 Gothenburg, Sweden
Titolo Testata:
JOURNAL OF MASS SPECTROMETRY
fascicolo: 6, volume: 36, anno: 2001,
pagine: 616 - 625
SICI:
1076-5174(200106)36:6<616:IOTNIP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MANGANESE-SUPEROXIDE-DISMUTASE; ELECTROSPRAY IONIZATION; PEPTIDE MIXTURES; OXIDATIVE STRESS; NITRIC-OXIDE; BRAIN-TISSUE; 3-NITROTYROSINE; PEROXYNITRITE; QUANTIFICATION; MARKER;
Keywords:
mass spectrometry; nitrotyrosine; peptides; protein; immonium ion; precursor ion scanning;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Roepstorff, P Univ So Denmark, Dept Biochem & Mol Biol, Odense Univ, DK-5230 Odense M, Denmark Univ So Denmark Odense Denmark M DK-5230 Odense M, Denmark
Citazione:
A.S. Petersson et al., "Investigation of tyrosine nitration in proteins by mass spectrometry", J MASS SPEC, 36(6), 2001, pp. 616-625

Abstract

In vivo nitration of tyrosine residues is a post-translational modification mediated by peroxynitrite that may be involved in a number of diseases. The aim of this study was to evaluate possibilities for sit e-specific detection of tyrosine nitration by mass spectrometry. Angiotensin II and bovine serum albumin (BSA) nitrated with tetranitromethane (TNM) were used as model compounds. Three strategies were investigated (i) analysis of single peptides and protein digests by matrix-assisted laser desorption/ionization (MALDI) peptide mass mapping, (ii) peptide mass mapping by electrospray ionization (ESI) mass spectrometry and (iii) screening for nitration by selectivedetection of the immonium ion of nitrotyrosine by precursor ion scanning with subsequent sequencing of the modified peptides. The MALDI time-of-flight mass spectrum of nitrated angiotensin II showed an unexpected prompt fragmentation involving the nitro group, in contrast to ESI-MS, where no fragmentation of nitrated angiotensin II was observed. The ESI mass spectra showed that mono- and dinitrated angiotensin II were obtained after treatment with TNM. ESI-MS/MS revealed that the mononitrated angiotensin II was nitrated on the side-chain of tyrosine. The dinitrated angiotensin II contained two nitro groups on the tyrosine residue. Nitration of BSA was confirmed by Western blotting with an antibody against nitrotyrosine and the sites for nitration were investigated by peptide mass mapping after in-gel digestion. Direct mass mapping by ESI revealed that two peptides were nitrated. Precursor ion scanning for the immonium ion for nitrotyrosine revealed two additional partially nitrated peptides. Based on the studies with the two model compounds, we suggest that the investigation of in ai oo nitration of tyrosine and identification of nitrated peptides might be performed by precursor ion scanning for the specific immonium ion at m/z 181.06 combined with ESI-MS/MS for identification of the specific nitration sites. copyright (C) 2001John Wiley & Sons, Ltd.

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Documento generato il 26/01/20 alle ore 09:55:14