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Titolo:
Signal transducers and activators of transcription (Stat) are detectable in mouse trigeminal ganglion neurons
Autore:
Kriesel, JD; Jones, BB; Hwang, IP; Dahms, KM; Spruance, SL;
Indirizzi:
Univ Utah, Med Ctr,Sch Med, Div Infect Dis, John A MOran Eye Ctr,Dept Ophthalmol, Salt Lake City, UT 84132 USA Univ Utah Salt Lake City UT USA 84132almol, Salt Lake City, UT 84132 USA Univ Utah, Sch Med, Dept Med, John A MOran Eye Ctr, Salt Lake City, UT 84132 USA Univ Utah Salt Lake City UT USA 84132 e Ctr, Salt Lake City, UT 84132 USA
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 6, volume: 21, anno: 2001,
pagine: 445 - 450
SICI:
1079-9907(200106)21:6<445:STAAOT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKEMIA INHIBITORY FACTOR; ACUTE-PHASE RESPONSE; NERVE GROWTH-FACTOR; TARGETED DISRUPTION; NEURITE OUTGROWTH; JAK-STAT; INTERLEUKIN-6; GP130; CELLS; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Kriesel, JD Univ Utah, Med Ctr,Sch Med, Div Infect Dis, John A MOran Eye Ctr,Dept Ophthalmol, Room 4B-322 SOM,50 N Med Dr, Salt Lake City, UT 84132 USA Univ Utah Room 4B-322 SOM,50 N Med Dr Salt Lake City UT USA 84132
Citazione:
J.D. Kriesel et al., "Signal transducers and activators of transcription (Stat) are detectable in mouse trigeminal ganglion neurons", J INTERF CY, 21(6), 2001, pp. 445-450

Abstract

We studied signal transducers and activators of transcription (Stat) expression in mouse trigeminal ganglia (TG) to gain an understanding of herpes simplex virus (HSV) infection and reactivation, Mouse TG were harvested and were either frozen for Western blot analysis or preserved in 4% paraformaldehyde for subsequent immunohistochemistry study. The thawed specimens were homogenized, and nuclear/cytoplasmic extractions were performed for Westernblots and immunoprecipitation. Immunohistochemistry showed that Stat1, Stat3. Stat4, Stat5b, and phosphotyrosine Stat3 localized to TG neurons, not surrounding satellite cells. Western blot of TG nuclear and cytoplasmic extracts confirmed the presence of these Stat at the appropriate molecular weights. Stat2 was undetectable in TG by these methods, Immunoprecipitation of TG nuclear extracts did not confirm the presence of Stat-Stat dimers in these specimens. These studies show that several Stat, including phosphotyrosine Stat3, are present in TG neurons, the site of HSV latency, where they could act upon latent viral DNA to effect reactivation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 04:58:13