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Titolo:
Lipopolysaccharide of Haemophilus influenzae induces interleukin-5 mRNA expression in human peripheral blood mononuclear cells
Autore:
Matsui, K; Tanaka, N; Nishikawa, A;
Indirizzi:
Meiji Pharmaceut Univ, Dept Immunobiol, Tokyo 2048588, Japan Meiji Pharmaceut Univ Tokyo Japan 2048588 munobiol, Tokyo 2048588, Japan
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 6, volume: 21, anno: 2001,
pagine: 439 - 443
SICI:
1079-9907(200106)21:6<439:LOHIII>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMOPHILUS-INFLUENZAE; HISTAMINE-RELEASE; CYCLOSPORINE-A; LUNG-DISEASE; T-CELLS; ENDOTOXIN; PROTEIN; BINDING; FK506; CD14;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Matsui, K Meiji Pharmaceut Univ, Dept Immunobiol, 2-522-1 Noshio, Tokyo 2048588, Japan Meiji Pharmaceut Univ 2-522-1 Noshio Tokyo Japan 2048588 Japan
Citazione:
K. Matsui et al., "Lipopolysaccharide of Haemophilus influenzae induces interleukin-5 mRNA expression in human peripheral blood mononuclear cells", J INTERF CY, 21(6), 2001, pp. 439-443

Abstract

Haemophilus influenzae is the bacterial species most often isolated from sputum of patients with chronic obstructive pulmonary disease (COPD), In this study, we examined the induction of interleukin-5 (IL-5) mRNA expression in human peripheral blood mononuclear cells (PBMC) stimulated with lipopolysaccharide (LPS) from H, influenzae to try to predict the effect of H, influenzae infection on the eosinophilic inflammation in COPD, Detection of IL-5 mRNA by RT-PCR showed that LPS from H, influenzae induced IL-5 mRNA expression in PBMC at a concentration of 1 mug/ml. Furthermore, the level of expression of IL-5 mRNA induced by LPS correlated with the amount of IL-5 protein in the culture supernatant. Inhibition of LPS-induced IL-5 mRNA expression by anti-CD14 antibody and diminution of this in a CD3(+)-cell-depleted fraction of PBMC, respectively, suggested that CD14 molecules were requiredfor the increase in IL-5 mRNA and that T lymphocytes were the principal source of IL-S mRNA expression in PBMC, Briefly, the IL-5 mRNA expression induced by LPS would be based on LPS-activated monocytes interacting with T lymphocytes to produce IL-5, These results may explain the role that colonization with H, influenzae plays in eosinophilic inflammation in patients withCOPD.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/10/20 alle ore 08:06:22