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Titolo:
Porcine caspase-3: Its cloning and activity during apoptosis of PK15 cellsinduced by porcine Fas ligand
Autore:
Muneta, Y; Shimoji, Y; Mori, Y;
Indirizzi:
Natl Inst Anim Hlth, Tsukuba, Ibaraki 3050856, Japan Natl Inst Anim Hlth Tsukuba Ibaraki Japan 3050856 Ibaraki 3050856, Japan
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 6, volume: 21, anno: 2001,
pagine: 409 - 415
SICI:
1079-9907(200106)21:6<409:PCICAA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERLEUKIN-1-BETA CONVERTING-ENZYME; IN-VIVO; MEDIATED APOPTOSIS; DEATH; INVOLVEMENT; LYMPHOCYTES; MECHANISMS; EXPRESSION; IL-1-BETA; PATHWAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Muneta, Y Natl Inst Anim Hlth, 3-1-5 Kannondai, Tsukuba, Ibaraki 3050856, Japan Natl Inst Anim Hlth 3-1-5 Kannondai Tsukuba Ibaraki Japan 3050856
Citazione:
Y. Muneta et al., "Porcine caspase-3: Its cloning and activity during apoptosis of PK15 cellsinduced by porcine Fas ligand", J INTERF CY, 21(6), 2001, pp. 409-415

Abstract

We cloned and sequenced cDNA that contained the coding sequence of porcinecaspase-3, The open reading frame (ORF) of porcine caspase-3 cDNA was 834 base pairs (bp) in length and encoded 277 amino acids. The predicted amino acid sequence was 88.4%, 86.6%, and 87.7% homologous to the predicted human, murine, and rat amino acid sequences, respectively. The activity of caspase-3 in porcine renal tubular cell line PK15 after recombinant porcine Fas ligand (FasL) stimulation was examined. The enzymatic activity of caspase-3, but not that of caspase-1, was significantly increased after FasL treatment. Western blot analysis also showed that the processing of caspase-3 fromproenzyme to mature subunits occurred after Fast treatment. The inhibitionof caspase-3 by its specific inhibitor partially prevented the apoptotic cell death of PK15 cells caused by Fast. The porcine caspase-3 cDNA isolatedin this study will be useful for the study of apoptotic cell death in pigsand will lead to the discovery of therapeutic uses: of caspases and their inhibitors in the prevention of viral and bacterial diseases and tissue injury associated with xenotransplantation and allotransplantation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 09:01:42