Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
An anti-inflammatory drug, propagermanium, may target GPI-anchored proteins associated with an MCP-1 receptor, CCR2
Autore:
Yokochi, S; Hashimoto, H; Ishiwata, Y; Shimokawa, H; Haino, M; Terashima, Y; Matsushima, K;
Indirizzi:
Sanwa Kagaku Kenkyusho Co Ltd, Cent Res Lab, Hokusei, Mie 5110406, Japan Sanwa Kagaku Kenkyusho Co Ltd Hokusei Mie Japan 5110406 ie 5110406, Japan Kyushu Univ, Grad Sch Med, Dept Cardiovasc Med, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ed, Higashi Ku, Fukuoka 8128582, Japan Univ Tokyo, CREST, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 o, CREST, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo, Sch Med, Dept Mol Prevent Med, Bunkyo Ku, Tokyo 1130033, JapanUniv Tokyo Tokyo Japan 1130033 vent Med, Bunkyo Ku, Tokyo 1130033, Japan
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 6, volume: 21, anno: 2001,
pagine: 389 - 398
SICI:
1079-9907(200106)21:6<389:AADPMT>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCYTE CHEMOATTRACTANT PROTEIN-1; DECAY-ACCELERATING FACTOR; ACTIVATING FACTOR MCAF/MCP-1; CHEMOKINE RECEPTORS; SIGNAL-TRANSDUCTION; TYROSINE KINASE; SMALL-MOLECULE; LIVER-INJURY; BINDING-SITE; T-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Ishiwata, Y Sanwa Kagaku Kenkyusho Co Ltd, Cent Res Lab, 363 Shiosaki, Hokusei, Mie 5110406, Japan Sanwa Kagaku Kenkyusho Co Ltd 363 Shiosaki HokuseiMie Japan 5110406
Citazione:
S. Yokochi et al., "An anti-inflammatory drug, propagermanium, may target GPI-anchored proteins associated with an MCP-1 receptor, CCR2", J INTERF CY, 21(6), 2001, pp. 389-398

Abstract

Monocyte chemoattractant protein-1 (MCP-1) promotes the migration and activation of monocytes and plays a pivotal role in the development of chronic inflammation. Propagermanium (3-oxygermylpropionic acid polymer) has been used as a therapeutic agent against chronic hepatitis B in Japan. We report here that propagermanium specifically inhibits in vitro chemotactic migration of monocytes by MCP-1, Propagermanium did not inhibit binding of MCP-1 to a human monocytic cell line, THP-1 cells, or affect intracellular Ca2+ mobilization or the cAMP concentration in MCP-1-treated THP-1 cells. The effect of propagermanium seems to require glycosylphosphatidylinositol (GPI)-anchored proteins, as cleavage of GPI anchors by phosphatidyl-inositol-phospholipase C (PI-PLC) eliminated the inhibitory activity of propagermanium, Anti-GPI-anchored protein antibodies, such as anti-CD55 and anti-CD59, reduced staining of C-C chemokine receptor 2 (CCR2) with an anti-CCR2 antibody against the N-terminus of CCR2 in a flow cytometric analysis, and these antibodies also selectively inhibited MCP-1-induced migration of THP-1 cells. Furthermore, under fluorescence microscopy, GPI-anchored proteins colocalizedwith CCR2 on THP-1 cells. These results suggest that propagermanium may target GPI-anchored proteins that are closely associated with CCR2 to selectively inhibit the MCP-1-induced chemotaxis, thus providing a mechanistic basis for the anti-inflammatory effects of the drug.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:55:15