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Titolo:
Hepatitis C virus RNA assays - A comparison of SuperQuant and Monitor
Autore:
Hadziyannis, E; Hadziyannis, A; Yen-Lieberman, B; Kiwi, ML; Hodnick, S; Spanou, F; Starkey, C; Younossi, ZM;
Indirizzi:
INOVA Fairfax Hosp, Ctr Liver Dis, Dept Med, Falls Church, VA 22042 USA INOVA Fairfax Hosp Falls Church VA USA 22042 , Falls Church, VA 22042 USA Hippokratio Gen Hosp, Acad Dept Med, Athens, Greece Hippokratio Gen Hosp Athens Greece Hosp, Acad Dept Med, Athens, Greece Hippokratio Gen Hosp, Natl Ctr Communicable Dis Liver, Athens, Greece Hippokratio Gen Hosp Athens Greece mmunicable Dis Liver, Athens, Greece Cleveland Clin Fdn, Dept Gastroenterol, Cleveland, OH 44195 USA Cleveland Clin Fdn Cleveland OH USA 44195 nterol, Cleveland, OH 44195 USA Cleveland Clin Fdn, Dept Clin Pathol, Cleveland, OH 44195 USA Cleveland Clin Fdn Cleveland OH USA 44195 Pathol, Cleveland, OH 44195 USA
Titolo Testata:
JOURNAL OF CLINICAL GASTROENTEROLOGY
fascicolo: 1, volume: 33, anno: 2001,
pagine: 45 - 48
SICI:
0192-0790(200107)33:1<45:HCVRA->2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRANCHED DNA; QUANTITATION; INTERFERON; VIREMIA; AMPLIFICATION; ALPHA;
Keywords:
HCV RNA; viral load; polymerase chain reaction quantification;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Younossi, ZM INOVA Fairfax Hosp, Ctr Liver Dis, Dept Med, 3300 Gallows Rd,Falls Church, VA 22042 USA INOVA Fairfax Hosp 3300 Gallows Rd Falls ChurchVA USA 22042
Citazione:
E. Hadziyannis et al., "Hepatitis C virus RNA assays - A comparison of SuperQuant and Monitor", J CLIN GAST, 33(1), 2001, pp. 45-48

Abstract

Hepatitis C RNA testing has been used extensively to assess the efficacy of antiviral therapy and has increasingly become an integral part of clinical management of patients with chronic hepatitis C. A variety of commercially available hepatitis C virus (HCV) RNA tests are used to detect HCV RNA qualitatively or quantitatively. These commercial tests have fundamental differences that are reflected on the values they generate. We compared two widely used assays, HCV SuperQuant (SQ) and Amplicor HCV Monitor (M1 and M2), in sera of patients with chronic hepatitis C. A total of 506 sera from 79 patients were tested with both assays. The data were logarithmically transformed and analyzed by linear regression and measurement of agreement. Two hundred thirty-eight sera had HCV RNA values within the dynamic range of bothassays. The correlation between the assays was fair, with a correlation coefficient (r) of 0.699. Overall, SQ generated higher values than M1 with a mean difference of 0.558 log (SD = 0.624). One hundred ninety-four (38%) and 121 (24%) of the sera were below the dynamic range of M1 and SQ, respectively. Seventy-three sera, undetectable by M1, were positive by SQ. The Amplicor HCV Monitor 2.0 (M2) was performed in 66 sera. All were positive by SQand M2, but only 38 were within the dynamic range of M1. The correlations between these tests were good (r = 0.68-0.78), but the agreement was ratherpoor. In conclusion, this study confirms that both SQ and M2 are more sensitive than M1. Additionally, our results show rather poor agreements between these assays. The recent attempts in standardizing the reporting of theseassays should make their results more easily interchangeable.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:37:31