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Titolo:
Sensitive and simple determination of mannitol in human brain tissues by gas chromatography-mass spectrometry
Autore:
Kiyoshima, A; Kudo, K; Hino, Y; Ikeda, N;
Indirizzi:
Kyushu Univ, Grad Sch Med Sci, Dept Forens Pathol & Sci, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 s Pathol & Sci, Fukuoka 8128582, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 758, anno: 2001,
pagine: 103 - 108
SICI:
1387-2273(20010705)758:1<103:SASDOM>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; PULSED AMPEROMETRIC DETECTION; INTESTINAL PERMEABILITY; SUGAR ALCOHOLS; SERUM; SORBITOL; URINE; GALACTITOL; LACTULOSE; SAMPLES;
Keywords:
mannitol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Ikeda, N Kyushu Univ, Grad Sch Med Sci, Dept Forens Pathol & Sci, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 & Sci, Fukuoka 8128582, Japan
Citazione:
A. Kiyoshima et al., "Sensitive and simple determination of mannitol in human brain tissues by gas chromatography-mass spectrometry", J CHROMAT B, 758(1), 2001, pp. 103-108

Abstract

A simple, reliable and sensitive gas chromatographic-mass spectrometric method was devised to determine the level of mannitol in various human brain tissues obtained at autopsy. Mannitol was extracted with 10% trichloroacetic acid solution which effectively precipitated brain tissues. The supernatant was washed with tert.-butyl methyl ether to remove other organic compounds acid to neutralize the aqueous solution. Mannitol was then derivatized with 1-butaneboronic acid and subjected to GC-MS. Erythritol was used as an internal standard. For quantitation, selected ion monitoring with m/z 127 and 253 for mannitol and m/z 127 for internal standard were: used. Calibration curves were linear in concentration range from 0.2 to 20 mug/0.1 g and correlation coefficients exceeded 0.99. The lower detection limit of mannitol in distilled water was 1 ng/0.1 g. Mannitol was detected in control braintissues, as a biological compound, at a level of 50 ng/0.1 g. The precision of this method was examined with use of two different concentrations, 2 and 20 mug/0.1 g, and the relative standard deviation ranged from 0.8 to 8.3%. We used this method to determine mannitol in brain tissues from an autopsied individual who had been clinically diagnosed as being brain dead. Cardiac arrest occurred 4 days later. (C) 2001 Elsevier Science B.V. All rightsreserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:33:36