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Titolo:
Experimental evidences and signal transduction pathways involved in the activation of NF-kappa B/Rel by angelan in murine macrophages
Autore:
Jeon, YJ; Kim, HM;
Indirizzi:
KRIBB, Taejon 305600, South Korea KRIBB Taejon South Korea 305600KRIBB, Taejon 305600, South Korea
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 7, volume: 1, anno: 2001,
pagine: 1331 - 1339
SICI:
1567-5769(200107)1:7<1331:EEASTP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTERIAL LIPOPOLYSACCHARIDE; GENE-EXPRESSION; DIFFERENTIAL ACTIVATION; PROTEIN-KINASES; INHIBITOR; LPS; PHOSPHORYLATION; TYROSINE; ELEMENT; C3H/HEJ;
Keywords:
Angelica gigas Nakai; angelan; macrophages; NF-kappa B/Rel; p38;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, HM KRIBB, POB 115,Yusong, Taejon 305600, South Korea KRIBB POB 115,Yusong Taejon South Korea 305600 05600, South Korea
Citazione:
Y.J. Jeon e H.M. Kim, "Experimental evidences and signal transduction pathways involved in the activation of NF-kappa B/Rel by angelan in murine macrophages", INT IMMUNO, 1(7), 2001, pp. 1331-1339

Abstract

In our previous studies. we showed that angelan, a polysaccharide purifiedfrom Angelica gigas Nakai, activated macrophages to induce the translocation of NF-kappa B/Rel into nucleus and DNA binding to its cognate site in the promoter of iNOS gene [Immunopharmacology 43 (1999) 1; Immunopharmacology49 (2000) 275]. In the present study, we showed that angelan induces the transcriptional activation of NF-kappaB/Rel and investigated the intracellular signal transduction pathways involved in the angelafi-induced NF-kappaB/Rel activation by murine macrophages. Treament of RAW 264.7 cells with angelan resulted in significant activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) and p38, while stress-activated protein kinase/c-JunNH2 terminal kinase (SAPK/JNK) was not activated by angelan. The specific p38 inhibitor SB203580 abrogated the angelan-induced NF-kappaB/Rel activation, whereas the selective MAPK/extracellular signal-regulated kinase 1 (MEK-1) inhibitor PD98059 did not affect the NF-kappaB/Rel induction. Treatmentof RAW 764.7 cells with both anti-CD14 Ab and anri-CR3 Ab significantly blocked angelan-induced NF-kappaB/Rel activation. In conclusion, we demonstrate that angelan induces NF-kappaB/Rel activation through the CD14 and CR3 membrane receptor and p38 kinase that is critically involved in the signal transduction leading to NF-kappaB/Rel activation in murine macrophages. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 22:21:24