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Titolo:
Similar effects of ether phospholipids, PAF and lyso-PAF on the Ca2+-ATPase activity of rat brain synaptosomes and leukocyte membranes
Autore:
Grosman, N;
Indirizzi:
Univ Copenhagen, Panum Inst, Dept Pharmacol, DK-2200 Copenhagen N, DenmarkUniv Copenhagen Copenhagen Denmark N acol, DK-2200 Copenhagen N, Denmark
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 7, volume: 1, anno: 2001,
pagine: 1321 - 1329
SICI:
1567-5769(200107)1:7<1321:SEOEPP>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLATELET-ACTIVATING-FACTOR; PROTEIN-KINASE-C; PLASMA-MEMBRANE; CALCIUM-PUMP; HUMAN NEUTROPHILS; MAST-CELLS; CA2+ PUMP; ATPASE; ANALOGS; MECHANISM;
Keywords:
plasma membrane Ca2+-ATPase activity; rat brain synaptosomes; rat leukocyte membranes; ET-16-OCH3; ET-18-OCH3 (edelfosine); D-20133; D-21266 (perifosine); PAF; lyso-PAF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Grosman, N Univ Copenhagen, Panum Inst, Dept Pharmacol, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark Univ Copenhagen Blegdamsvej 3 Copenhagen Denmark N N, Denmark
Citazione:
N. Grosman, "Similar effects of ether phospholipids, PAF and lyso-PAF on the Ca2+-ATPase activity of rat brain synaptosomes and leukocyte membranes", INT IMMUNO, 1(7), 2001, pp. 1321-1329

Abstract

The present study is an extension of our previous work with the antineoplastic ether phospholipid ET-IS-OCH, (edelfosine), which was shown to affect the activity of the Ca2+-ATPase of rat brain synaptosomes and peritoneal leukocyte membranes. The effect of ET-18-OCH3 was compared with that of the 16-carbon chain analogue ET-16-OCH3 as well as with the structurally related16- and 18-carbon PAFs (platelet-activating factors) and lyso-PAFs. In addition, the two alkylphosphocholines D-20166 and D-21266 (perifosine) were included in the investigation. The influence of all of the compounds followed the same pattern, i.e., the Ca2+-ATPase activity of the synaptosomes was increased over a relatively narrow concentration range (peak at 20-30 muM) and that of the leukocyte membranes was inhibited in a concentration-dependent manner by 10-50 muM concentrations of the drugs. Ether phospholipids with an 18-carbon chain at C-1 were more potent than those with a Ih-carbon chain. All of the compounds increased the activity of the synaptosomal ATPase to the same extend (ca. 50%). With the exception of lyso-PAF, all inhibited the enzyme activity of leukocyte membranes by 60-70%, whereas lyso-PAF was less effective (ca. 50% inhibition). The concentration range of activityfur PAF and lyso-PAF indicates that their effect on the enzyme activity was caused by receptor-independent mechanisms. The ether phospholipids and alkylphosphocholines are suggested to act by accumulating in the membranes and thereby altering the character of the lipid environment of the enzyme rather than by a direct interaction with the Ca2+-ATPase. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 25/01/20 alle ore 04:02:43