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Titolo:
Activation-induced T cell apoptosis by monocytes from stem cell products
Autore:
Ino, K; Ageitos, AG; Singh, RK; Talmadge, JE;
Indirizzi:
Univ Nebraska, Med Ctr, Dept Pathol & Microbiol, Omaha, NE 68198 USA Univ Nebraska Omaha NE USA 68198 Pathol & Microbiol, Omaha, NE 68198 USA Fundac Jimenez Diaz, Serv Oncol, Dept Oncol, Madrid 28015, Spain Fundac Jimenez Diaz Madrid Spain 28015 , Dept Oncol, Madrid 28015, Spain Nagoya Univ, Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan Nagoya Univ Nagoya Aichi Japan 4668550 a Ku, Nagoya, Aichi 4668550, Japan
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 7, volume: 1, anno: 2001,
pagine: 1307 - 1319
SICI:
1567-5769(200107)1:7<1307:ATCABM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-DOSE CHEMOTHERAPY; BONE-MARROW TRANSPLANTATION; COLONY-STIMULATING FACTOR; IMMUNE RECONSTITUTION; NITRIC-OXIDE; GM-CSF; SUPPRESSOR CELLS; NK-CELL; IMMUNOLOGICAL RECONSTITUTION; CANCER PATIENTS;
Keywords:
monocytes; peripheral tolerance; T cells; IL-2; mitogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Talmadge, JE Univ Nebraska, Med Ctr, Dept Pathol & Microbiol, 987660 Nebraska Med Ctr, Omaha, NE 68198 USA Univ Nebraska 987660 Nebraska Med Ctr Omaha NE USA 68198 USA
Citazione:
K. Ino et al., "Activation-induced T cell apoptosis by monocytes from stem cell products", INT IMMUNO, 1(7), 2001, pp. 1307-1319

Abstract

We recently found that mobilized peripheral blood stem cell (PSC) products(from both cancer patients and normal donors) contain high levels of CD14() monocytes, which can inhibit the proliferation of allogeneic and autologous T cells. We found in our studies that using CD14(+) monocytes from mobilized PSC products (from normal and cancer patient donors), normal apheresis products or normal peripheral blood (PB) can affect lymphocyte function and apoptosis-dependent T cell activation. However, it appears that the apoptosis is dependent on the frequency of monocytes, which is: increased by both mobilization and apheresis. Both phytohemagglutinin (PHA)- and interleukin (IL)-2-induced proliferation of steady-state peripheral blood mononuclear cells (PBMC) were markedly inhibited by co-culture with irradiated CD14(+) monocytes, although inhibition was significantly greater with PHA than with IL-2 stimulation. IL-2 (predominately CD56(+) NK cells) or anti-CD3 monoclonal antibody (mAb) and IL-2-expanded lymphocytes (activated T cells) were inhibited by PSC monocytes to a significantly greater level as compared to steady-state lymphocytes. Indeed, no inhibition of T cell proliferation was observed when lymphocytes were co-cultured in the absence of mitogenic or IL-2 stimulation. In contrast. an increased proliferation was observed inco-cultures of CD14(+) monocytes and steady-state or activated lymphocyteswithout mitogenic stimulation. Cell cycle analysis by flow cytometry revealed a significant increase in hypodiploid DNA, in a time-dependent manner, following co-culture of monocytes and PBMC in PHA, suggesting that T cell apoptosis occurred during PHA-induced activation, These results demonstrate that PSC-derived monocytes inhibit T cell proliferation by inducing the apoptosis of activated T cells and Nk: cells. but not steady-state cells. Thissuggests a potential role for monocytes in the induction of peripheral tolerance following stem cell transplantation, (C) 2001 Elsevier Science B,V. All rights reserved.

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Documento generato il 19/01/20 alle ore 20:45:34