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Titolo:
Activation of murine cytomegalovirus immediate-early promoter in cerebral ventricular zone and glial progenitor cells in transgenic mice
Autore:
Li, RY; Baba, S; Kosugi, I; Arai, Y; Kawasaki, H; Shinmura, Y; Sakakibara, S; Okano, H; Tsutsui, Y;
Indirizzi:
Hamamatsu Univ, Sch Med, Dept Pathol 2, Hamamatsu, Shizuoka 4313192, JapanHamamatsu Univ Hamamatsu Shizuoka Japan 4313192 , Shizuoka 4313192, Japan Keio Univ, Sch Med, Dept Physiol, Shinjuku Ku, Tokyo 160, Japan Keio UnivTokyo Japan 160 d, Dept Physiol, Shinjuku Ku, Tokyo 160, Japan
Titolo Testata:
GLIA
fascicolo: 1, volume: 35, anno: 2001,
pagine: 41 - 52
SICI:
0894-1491(200107)35:1<41:AOMCIP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; ASTROCYTE-SPECIFIC EXPRESSION; STEM-CELLS; VIRUS INFECTION; MAMMALIAN CNS; MOUSE EMBRYO; BRAIN; NEURONS; DISORDERS; PROTEIN;
Keywords:
congenital infection; brain abnormalities; neural stem cell; glial differentiation; Musashi1 gene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Tsutsui, Y Hamamatsu Univ, Sch Med, Dept Pathol 2, 1-20-1 Handayama, Hamamatsu, Shizuoka 4313192, Japan Hamamatsu Univ 1-20-1 Handayama Hamamatsu Shizuoka Japan 4313192
Citazione:
R.Y. Li et al., "Activation of murine cytomegalovirus immediate-early promoter in cerebral ventricular zone and glial progenitor cells in transgenic mice", GLIA, 35(1), 2001, pp. 41-52

Abstract

Cytomegalovirus (CMV) is the most common infectious cause of congenital anomalies of the CNS in humans. We recently reported that the murine cytomegalovirus (MCMV) immediate-early (IE) gene promoter directs astrocyte-specific expression in adult transgenic mice. In the present study, we analyzed the activation of the MCMV IE promoter in developing transgenic mouse brains and compared the activation with that of the Musashi 1 (Msi1) gene, which is expressed in neural progenitor cells, including neural stem cells. Duringthe early phase of neurogenesis, the transgene was expressed predominantlyin endothelial cells of the vessels, but not in neuroepithelial cells in which Msi1 was expressed. During later stages of gestation, expression of the transgene was largely restricted to the ventricular zone (VZ) in the CNS,similar to the expression of Msi1. In neurosphere cultures from transgenicembryos in the late phase of neurogenesis, the transgene was expressed in some cells of neurospheres expressing Msi1 and nestin. In neural precursor cells induced to differentiate from stem cells, expression of the transgenewas detected in glial progenitor cells, expressing GFAP, nestin, and Msi1,but not in cells expressing MAP2 or MAG. In postnatal development, persistent expression of the transgene was observed in astrocyte lineage cells as was Msi1. These spatiotemporal changes of the MCMV LE promoter activity during development of transgenic mice correlated with susceptible sites in congenital HCMV infection. Moreover, this transgenic mouse model may provide useful model for analysis of the regulation of the switching of neuronal andastrocyte differentiation, and the maintenance of the astrocyte lineage. GLIA 35:41-52, 2001. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 09/04/20 alle ore 00:09:49