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Titolo:
Role of the extracellular matrix in myelination of peripheral nerve
Autore:
Podratz, JL; Rodriguez, E; Windebank, AJ;
Indirizzi:
Mayo Grad Sch & Med Sch, Mol Neurosci Program, Rochester, MN USA Mayo GradSch & Med Sch Rochester MN USA osci Program, Rochester, MN USA
Titolo Testata:
GLIA
fascicolo: 1, volume: 35, anno: 2001,
pagine: 35 - 40
SICI:
0894-1491(200107)35:1<35:ROTEMI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCHWANN-CELLS INVITRO; BASAL LAMINA; DIFFERENTIATION; BIOSYNTHESIS; EXPRESSION; COLLAGEN; GLYCOPROTEIN; CULTURES; AXONS; GENE;
Keywords:
myelin; laminin; basal lamina; integrin receptor; Schwann cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Windebank, AJ Mayo Clin & Mayo Fdn, 1501 Guggenheim Bldg,200 1st St SW, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn 1501 Guggenheim Bldg,200 1st St SW Rochester MN USA 55905
Citazione:
J.L. Podratz et al., "Role of the extracellular matrix in myelination of peripheral nerve", GLIA, 35(1), 2001, pp. 35-40

Abstract

Assembly of the extracellular matrix (ECM) has been tightly linked to compact myelin formation in the peripheral nervous system. We recently demonstrated that myelination of dorsal root ganglion (DRG) axons by Schwann cells may occur in the absence of basal lamina. We have now determined whether laminin deposition occurs around myelinating SC, even though basal lamina hasnot been assembled. DRG/SC co-cultures were prepared from E15 rat embryos and incubated in fully defined medium (B27) with and without ascorbic acid for 21-24 days. Cultures were stained with a rabbit anti-laminin antibody and examined by laser confocal fluorescence microscopy. Myelination occurredin both groups. In the presence of ascorbic acid, there was dense even laminin staining around myelinating SC. In the absence of ascorbic acid, laminin staining was also present but was irregular and less dense. DRG and SC were cocultured without ascorbic acid in the presence or absence of a function blocking anti-beta (1) integrin receptor antibody. The antibody completely inhibited myelination. Finally, DRG/SC co-cultures were prepared both with and without ascorbic acid and incubated under control conditions or in the presence of continual, gentle motion. Movement in the absence of ECM significantly inhibited myelination. This demonstrates that laminin depositionon the surface of SC but not ECM assembly is required for formation of compact myelin. ECM is required to provide mechanical stability during the process of myelination. GLIA 35:35-40, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:08:09