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Titolo:
Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons
Autore:
Sasaki, M; Honmou, O; Akiyama, Y; Uede, T; Hashi, K; Kocsis, JD;
Indirizzi:
Sapporo Med Univ, Sch Med, Dept Neurosurg, Chuo Ku, Sapporo, Hokkaido 0608543, Japan Sapporo Med Univ Sapporo Hokkaido Japan 0608543 , Hokkaido 0608543, Japan Yale Univ, Sch Med, Dept Neurol, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 ed, Dept Neurol, New Haven, CT 06510 USA Vet Adm Med Ctr, Neurosci Res Ctr, W Haven, CT USA Vet Adm Med Ctr W Haven CT USA ed Ctr, Neurosci Res Ctr, W Haven, CT USA
Titolo Testata:
GLIA
fascicolo: 1, volume: 35, anno: 2001,
pagine: 26 - 34
SICI:
0894-1491(200107)35:1<26:TOAAIB>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; NEURAL STEM-CELLS; MYELINATION FOLLOWING TRANSPLANTATION; PROGENITOR CELLS; STROMAL CELLS; SCHWANN-CELLS; MAMMALIAN FOREBRAIN; DEFICIENT RAT; IN-VIVO; BRAIN;
Keywords:
axon; bone marrow; demyelination; glia; myelin; multiple sclerosis; Schwann cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Honmou, O Sapporo Med Univ, Sch Med, Dept Neurosurg, Chuo Ku, South 1st,West 16th, Sapporo, Hokkaido 0608543, Japan Sapporo Med Univ South 1st,West 16th Sapporo Hokkaido Japan 0608543
Citazione:
M. Sasaki et al., "Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons", GLIA, 35(1), 2001, pp. 26-34

Abstract

The potential of bone marrow cells to differentiate into myelin-forming cells and to repair the demyelinated rat spinal cord in vivo was studied using cell transplantation techniques. The dorsal funiculus of the spinal cord was demyelinated by x-irradiation treatment, followed by microinjection of ethidium bromide. Suspensions of a bone marrow cell fraction acutely isolated from femoral bones in LacZ transgenic mice were prepared by centrifugation on a density gradient (Ficoll-Paque) to remove erythrocytes, platelets, and debris. The isolated cell fraction contained hematopoietic and nonhematopoietic stem and precursor cells and lymphocytes. The cells were transplanted into the demyelinated dorsal column lesions of immunosuppressed rats. An intense blue beta -galactosidase reaction was observed in the transplantation zone. The genetically labeled bone marrow cells remyelinated the spinal cord with predominately a peripheral pattern of myelination reminiscent of Schwann cell myelination. Transplantation of CD34(+) hematopoietic stem cells survived in the lesion, but did not form myelin. These results indicate that bone marrow cells can differentiate in vivo into myelin-forming cells and repair demyelinated CNS. GLIA 35:26-34, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 06:32:40