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Titolo:
Characterization of alpha-casozepine, a tryptic peptide from bovine alpha(s1)-casein with benzodiazepine-like activity
Autore:
Miclo, L; Perrin, E; Driou, A; Papadopoulos, V; Boujrad, N; Vanderesse, R; Boudier, JF; Desor, D; Linden, G; Gaillard, JL;
Indirizzi:
Univ Nancy 1, Fac Sci, Lab Biosci Aliment, INRA,UA 885, F-54506 VandoeuvreNancy, France Univ Nancy 1 Vandoeuvre Nancy France F-54506 506 VandoeuvreNancy, France Georgetown Univ, Med Ctr, Dept Cell Biol, Washington, DC 20007 USA Georgetown Univ Washington DC USA 20007 ll Biol, Washington, DC 20007 USA Georgetown Univ, Med Ctr, Dept Pharmacol, Washington, DC 20007 USA Georgetown Univ Washington DC USA 20007 armacol, Washington, DC 20007 USA Georgetown Univ, Med Ctr, Dept Neurosci, Washington, DC 20007 USA Georgetown Univ Washington DC USA 20007 eurosci, Washington, DC 20007 USA INPL, ENSIC, Lab Chim Phys Macromol, CNRS,UMR 7568, Nancy, France INPL Nancy France Lab Chim Phys Macromol, CNRS,UMR 7568, Nancy, France Ingredia, Arras, France Ingredia Arras FranceIngredia, Arras, France Univ Nancy 1, Fac Sci, Lab Aspects Fonct & Dev Comportements, CNRS,URA 1293, F-54506 Vandoeuvre Nancy, France Univ Nancy 1 Vandoeuvre Nancy France F-54506 06 Vandoeuvre Nancy, France Univ Rennes 1, Endocrinol Mol Reprod UMR CNRS Interact Cellulair, F-35042 Rennes, France Univ Rennes 1 Rennes France F-35042 ct Cellulair, F-35042 Rennes, France
Titolo Testata:
FASEB JOURNAL
fascicolo: 8, volume: 15, anno: 2001,
pagine: NIL_466 - NIL_487
SICI:
0892-6638(200106)15:8<NIL_466:COAATP>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELEVATED PLUS-MAZE; BINDING INHIBITOR RECEPTOR; IMMUNOREACTIVE MATERIAL; LIQUID-CHROMATOGRAPHY; ANXIOGENIC ACTION; KINDLED SEIZURES; MILK-PROTEINS; PENTYLENETETRAZOL; DIAZEPAM; RATS;
Keywords:
milk; casein peptide; anxiolysis; anticonvulsant; diazepam binding inhibitor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Miclo, L Univ Nancy 1, Fac Sci, Lab Biosci Aliment, INRA,UA 885, BP 239, F-54506 Vandoeuvre Nancy, France Univ Nancy 1 BP 239 Vandoeuvre Nancy France F-54506 ancy, France
Citazione:
L. Miclo et al., "Characterization of alpha-casozepine, a tryptic peptide from bovine alpha(s1)-casein with benzodiazepine-like activity", FASEB J, 15(8), 2001, pp. NIL_466-NIL_487

Abstract

Caseins are a known source of biologically active peptides. In this study,we have shown evidence of a novel anxiolytic activity in a tryptic hydrolysate of bovine alpha (s1)-casein. Injection of 3 mg/kg of this hydrolysate significantly reduced the epileptic symptoms caused by pentylenetetrazole in rats. Anxiety reduction was also observed when the hydrolysate was testedin the elevated plus-maze and in the conditioned defensive burying rat models. Peptides isolated from the hydrolysate were examined for their affinity for the gamma -amino-butyric acid (GABA) type A receptor. Only one peptide, named alpha -casozepine, corresponding to the 91-100 fragment from bovine alpha (s1)-casein, expressed affinity for GABA(A) receptor. In vitro, thepeptide had 10,000 less affinity for the benzodiazepine site of the GABA(A) than did diazepam. However, in the conditioned defensive burying paradigmit was 10-fold more efficient than diazepam. The difference observed between the in vitro and in vivo activity of alpha -casozepine could not been explained by an action via the peripheral-type benzodiazepine receptor; alpha-casozepine had no affinity for this receptor. The alpha -casozepine aminoacid sequence could be related to the carboxyterminal sequence of the polypeptide diazepam binding inhibitor, an endogenous ligand of the central GABA(A) and peripheral-type benzodiazepine receptors.

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Documento generato il 23/01/20 alle ore 03:56:05