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Titolo:
Insulin and glucocorticoids differentially regulate leptin transcription and secretion in brown adipocytes
Autore:
Buyse, M; Viengchareun, S; Bado, A; Lombes, M;
Indirizzi:
Fac Med Xavier Bichat, Inst Federatif Rech Cellules Epitheliales, INSERM, U478, F-75870 Paris 18, France Fac Med Xavier Bichat Paris France 18 RM, U478, F-75870 Paris 18, France Fac Med Xavier Bichat, Inst Federatif Rech Cellules Epitheliales, INSERM, U410, F-75870 Paris, France Fac Med Xavier Bichat Paris France F-75870 , U410, F-75870 Paris, France
Titolo Testata:
FASEB JOURNAL
fascicolo: 8, volume: 15, anno: 2001,
pagine: 1357 - 1366
SICI:
0892-6638(200106)15:8<1357:IAGDRL>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
OB GENE-EXPRESSION; WHITE ADIPOSE-TISSUE; BINDING-PROTEIN-ALPHA; MOUSE OBESE GENE; 3T3-L1 ADIPOCYTES; RAT ADIPOCYTES; MESSENGER-RNA; UNCOUPLING PROTEIN; PLASMA LEPTIN; FOOD-INTAKE;
Keywords:
cell line; uncoupling protein; ob gene; signaling pathway; obesity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Lombes, M Fac Med Xavier Bichat, Inst Federatif Rech Cellules Epitheliales, INSERM, U478, IFR2,16 Rue Henri Huchard, F-75870 Paris 18, France Fac MedXavier Bichat IFR2,16 Rue Henri Huchard Paris France 18
Citazione:
M. Buyse et al., "Insulin and glucocorticoids differentially regulate leptin transcription and secretion in brown adipocytes", FASEB J, 15(8), 2001, pp. 1357-1366

Abstract

Leptin, the ob gene product, is produced by adipose tissue and is submitted to a complex hormonal and metabolic regulation. Leptin plays a critical role in the balance of body weight. Here we report on secretion and hormonalregulation of leptin by brown adipocytes. Using the recently established T37i cell line, we show that leptin expression and secretion occurred as a function of cell differentiation. In differentiated T37i cells, insulin induced leptin release (similar to0.25 ng/10(6) cells/h) in a concentration-dependent manner (EC50=0.1 nM), and this was totally suppressed by beta3 -adrenergic ligand, thiazolidinedione, cycloheximide, or actinomycin D. Insulin induced a strong, rapid (within 2 h) but transient fivefold increase in leptin mRNA levels. This transcriptional control of ob gene expression by insulin involved both phosphatidylinositol 3-kinase- and MAP kinase-dependent pathways. Glucocorticoids inhibited both insulin-stimulated leptin secretionand ob gene expression without affecting leptin mRNA stability (t(1/2)=3h05). Altogether, our results demonstrate that brown adipocytes express and secrete leptin, whose hormonal regulation clearly differs from that described in white adipose tissue. These findings point to tissue-specific molecular mechanisms and suggest that leptin might exert direct effects on energy homeostasis through an autocrine mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 00:28:47